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Proximity labeling proteomics with cilia-TurboID transgenic mice identified regulators of motile cilia function

doi: 10.1016/j.jgg.2025.07.013
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Henan Key Laboratory of Population Defects Prevention (ZD202302).

This work was supported by grants from the National Key Research and Development Program of China (2019YFA0802704), the National Natural Science Foundation of China (31771620), the Natural Science Foundation of Chongqing, China (CSTB2022NSCQ-MSX1424), Research Startup Fund of Southwest University (SWU117064), and Open Research Fund of National Health Commission Key Laboratory of Birth Defects Prevention &

  • Received Date: 2025-01-22
  • Accepted Date: 2025-07-28
  • Rev Recd Date: 2025-07-28
  • Available Online: 2025-08-06
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  • Cho, K.F., Branon, T.C., Udeshi, N.D., Myers, S.A., Carr, S.A., Ting, A.Y., 2020. Proximity labeling in mammalian cells with TurboID and split-TurboID. Nat. Protoc. 15, 3971-3999.
    Fernandez-Gonzalez, A., Kourembanas, S., Wyatt, T.A., Mitsialis, S.A., 2009. Mutation of murine adenylate kinase 7 underlies a primary ciliary dyskinesia phenotype. Am. J. Respir. Cell Mol. Biol. 40, 305-313.
    Ishikawa, H., Thompson, J., Yates, J.R., 3rd, Marshall, W.F., 2012. Proteomic analysis of mammalian primary cilia. Curr. Biol. 22, 414-419.
    Liu, X., Yam, P.T., Schlienger, S., Cai, E., Zhang, J., Chen, W.J., Torres Gutierrez, O., Jimenez Amilburu, V., Ramamurthy, V., Ting, A.Y., et al., 2024. Numb positively regulates Hedgehog signaling at the ciliary pocket. Nat. Commun. 15, 3365.
    Loukil, A., Ebright, E., Hamdi, K., Menzel, E., Uezu, A., Gao, Y., Soderling, S.H., Goetz, S.C., 2025. Identification of new ciliary signaling pathways in the brain and insights into neurological disorders. J. Neurosci. https://doi.org/10.1523/JNEUROSCI.0800-24.2025.
    Martino, F., Pal, M., Munoz-Hernandez, H., Rodriguez, C.F., Nunez-Ramirez, R., Gil-Carton, D., Degliesposti, G., Skehel, J.M., Roe, S.M., Prodromou, C., et al., 2018. RPAP3 provides a flexible scaffold for coupling HSP90 to the human R2TP co-chaperone complex. Nat. Commun. 9, 1501.
    Mick, D.U., Rodrigues, R.B., Leib, R.D., Adams, C.M., Chien, A.S., Gygi, S.P., Nachury, M.V., 2015. Proteomics of primary cilia by proximity labeling. Dev. Cell 35, 497-512.
    Murata, K., Mimura, A., Suzuki, H., Mikami, N., Hamada, Y., Kato, K., Iki, N., Ishida, M., Daitoku, Y., Tanimoto, Y., et al., 2021. Efficient induction of proximity-dependent labelling by biotin feeding in BMAL1-BioID knock-in mice. J. Biochem. 170, 453-461.
    Reiter, J.F., Leroux, M.R., 2017. Genes and molecular pathways underpinning ciliopathies. Nat. Rev. Mol. Cell Biol. 18, 533-547.
    Sheridan, J., Grata, A., Dorr, J., Suva, E.E., Bresteau, E., Mitchell, L.R., Hassan, O., Mitchell, B., 2025. Centriolar defects underlie a primary ciliary dyskinesia phenotype in an adenylate kinase 7 deficient ciliated epithelium. Dev. Biol. 524, 152-161.
    Sigg, M.A., Menchen, T., Lee, C., Johnson, J., Jungnickel, M.K., Choksi, S.P., Garcia, G., Busengdal, H., Dougherty, G.W., Pennekamp, P., 2017. Evolutionary proteomics uncovers ancient associations of cilia with signaling pathways. Dev. Cell 43, 744-762.
    Wallmeier, J., Nielsen, K.G., Kuehni, C.E., Lucas, J.S., Leigh, M.W., Zariwala, M.A., Omran, H., 2020. Motile ciliopathies. Nat. Rev. Dis. Primers 6, 77.
    Wu, H., Zhang, Y., Li, Y., Sun, S., Zhang, J., Xie, Q., Dong, Y., Zhou, S., Sha, X., Li, K., et al., 2024. Adenylate kinase phosphate energy shuttle underlies energetic communication in flagellar axonemes. Sci. China Life Sci. 67, 1697-1714.
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