Stomatal maturomics: hunting genes regulating guard cell maturation and function formation from single-cell transcriptomes

Yuming Peng; Yi Liu; Yifan Wang; Zhenxing Geng; Yue Qin; Shisong Ma

doi:10.1016/j.jgg.2024.05.004

Volume 51 Issue 11

Nov. 2024

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Article Navigation > Journal of Genetics and Genomics > 2024 > 51(11): 1286-1299

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Stomatal maturomics: hunting genes regulating guard cell maturation and function formation from single-cell transcriptomes

doi: 10.1016/j.jgg.2024.05.004

a. MOE Key Laboratory for Cellular Dynamics, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Innovation Academy for Seed Design, Chinese Academy of Sciences, Hefei, Anhui 230027, China;
b. School of Data Science, University of Science and Technology of China, Hefei, Anhui 230027, China

Funds:

This work was supported by grants from the Strategic Priority Research Program of the Chinese Academy of Science (XDA24010303), the National Natural Science Foundation of China (31770268), the Fundamental Research Funds for the Central Universities (WK2070000091), and the University of Science and Technology of China (Start-up fund to S.M.).

Received Date: 2024-05-14
Accepted Date: 2024-05-17
Rev Recd Date: 2024-05-17

Available Online: 2025-06-06

Publish Date: 2024-05-19

Abstract

Abstract

Stomata play critical roles in gas exchange and immunity to pathogens. While many genes regulating early stomatal development up to the production of young guard cells (GCs) have been identified in Arabidopsis, much less is known about how young GCs develop into mature functional stomata. Here we perform a maturomics study on stomata, with “maturomics” defined as omics analysis of the maturation process of a tissue or organ. We develop an integrative scheme to analyze three public stomata-related single-cell RNA-seq datasets and identify a list of 586 genes that are specifically up-regulated in all three datasets during stomatal maturation and function formation. The list, termed sc_586, is enriched with known regulators of stomatal maturation and functions. To validate the reliability of the dataset, we selected two candidate G2-like transcription factor genes, MYS1 and MYS2, to investigate their roles in stomata. These two genes redundantly regulate the size and hoop rigidity of mature GCs, and the mys1 mys2 double mutants cause mature GCs with severe defects in regulating their stomatal apertures. Taken together, our results provide a valuable list of genes for studying GC maturation and function formation.
- Stomata maturomics,
- Hoop rigidity,
- Stomatal aperture,
- Single-cell analysis,
- G2-like transcription factors,
- MYS1,
- MYS2

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