Two ABCI family transporters, OsABCI15 and OsABCI16, are involved in grain-filling in rice

Bin Ma; Xiubiao Cao; Xiaoyuan Li; Zhong Bian; Qi-Qi Zhang; Zijun Fang; Jiyun Liu; Qun Li; Qiaoquan Liu; Lin Zhang; Zuhua He

doi:10.1016/j.jgg.2023.10.007

Volume 51 Issue 5

May 2024

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Article Navigation > Journal of Genetics and Genomics > 2024 > 51(5): 492-506

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Two ABCI family transporters, OsABCI15 and OsABCI16, are involved in grain-filling in rice

doi: 10.1016/j.jgg.2023.10.007

a. Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology, Agricultural College of Yangzhou University, Yangzhou, Jiangsu 225009, China;
b. Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou, Jiangsu 225009, China;
c. National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology & Ecology, Chinese Academy of Sciences, Shanghai 200032, China;
d. Institute of Biotechnology, Hangzhou Academy of Agricultural Sciences, Hangzhou, Zhejiang 310024, China;
e. Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Yangzhou University, Yangzhou, Jiangsu 225009, China

Funds:

The work was supported by the National Natural Science Foundation of China (32100206 and 32072037), the Research Programs from Jiangsu Government (BE2022336), and the Project of Zhongshan Biological Breeding Laboratory (BM2022008-02). This work was also supported the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) and the Key Scientific Research Project of the Higher Education Institution in Jiangsu Province (No. 20KJA210002).

Received Date: 2023-10-07
Accepted Date: 2023-10-27
Rev Recd Date: 2023-10-27

Available Online: 2025-06-06

Publish Date: 2023-10-31

Abstract

Abstract

Seed development is critical for plant reproduction and crop yield, with panicle seed-setting rate, grain-filling, and grain weight being key seed characteristics for yield improvement. However, few genes are known to regulate grain filling. Here, we identify two adenosine triphosphate (ATP)-binding cassette (ABC)I-type transporter genes, OsABCI15 and OsABCI16, involved in rice grain-filling. Both genes are highly expressed in developing seeds, and their proteins are localized to the plasma membrane and cytosol. Interestingly, knockout of OsABCI15 and OsABCI16 results in a significant reduction in seed-setting rate, caused predominantly by the severe empty pericarp phenotype, which differs from the previously reported low seed-setting phenotype resulting from failed pollination. Further analysis indicates that OsABCI15 and OsABCI16 participate in ion homeostasis and likely export ions between filial tissues and maternal tissues during grain filling. Importantly, overexpression of OsABCI15 and OsABCI16 enhances the seed-setting rate and grain yield in transgenic plants and decreases ion accumulation in brown rice. Moreover, the OsABCI15/16 orthologues in maize exhibit a similar role in kernel development, as demonstrated by their disruption in transgenic maize. Therefore, our findings reveal the important roles of two ABC transporters in cereal grain filling, highlighting their value in crop yield improvement.
- ABC transporters,
- Rice,
- Grain filling,
- Ion homeostasis,
- Grain yield

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References

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