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Volume 50 Issue 12
Dec.  2023
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Article Navigation >  Journal of Genetics and Genomics  > 2023  >  50(12): 983-992

PIFs interact with SWI2/SNF2-related 1 complex subunit 6 to regulate H2A.Z deposition and photomorphogenesis in Arabidopsis

doi: 10.1016/j.jgg.2023.04.008
  •  

    a. Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China;

  •  

    b. School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China

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This work was supported by the National Natural Science Foundation of China (31900609), the National Key Research and Development Program of China (2017YFA0503802), the National Natural Science Foundation of China (31530085, 31900207, and 32000183), and the Science and Technology Commission of Shanghai Municipality (18DZ2260500).

  • Received Date: 2023-03-08
  • Accepted Date: 2023-04-18
  • Rev Recd Date: 2023-04-15
  • Publish Date: 2023-04-28
    Abstract
  • Light is an essential environmental signal perceived by a broad range of photoreceptors in plants. Among them, the red/far-red light receptor phytochromes function to promote photomorphogenesis, which is critical to the survival of seedlings after seeds germination. The basic-helix-loop-helix transcription factors phytochrome-interacting factors (PIFs) are the pivotal direct downstream components of phytochromes. H2A.Z is a highly conserved histone variant regulating gene transcription, and its incorporation into nucleosomes is catalyzed by SWI2/SNF2-related 1 complex, in which SWI2/SNF2-related 1 complex subunit 6 (SWC6) and actin-related protein 6 (ARP6) serve as core subunits. Here, we show that PIFs physically interact with SWC6 in vitro and in vivo, leading to the disassociation of HY5 from SWC6. SWC6 and ARP6 regulate hypocotyl elongation partly through PIFs in red light. PIFs and SWC6 coregulate the expression of auxin-responsive genes such as IAA6, IAA19, IAA20, and IAA29 and repress H2A.Z deposition at IAA6 and IAA19 in red light. Based on previous studies and our findings, we propose that PIFs inhibit photomorphogenesis, at least in part, through repression of H2A.Z deposition at auxin-responsive genes mediated by the interactions of PIFs with SWC6 and promotion of their expression in red light.
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