Fecal microbiota transplantation from young donor mice improves ovarian function in aged mice

Li Xu; Qiankun Zhang; Xiaowei Dou; Yipeng Wang; Jianwei Wang; Yong Zhou; Xingyin Liu; Jing Li

doi:10.1016/j.jgg.2022.05.006

Volume 49 Issue 11

Nov. 2022

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Article Navigation > Journal of Genetics and Genomics > 2022 > 49(11): 1042-1052

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Fecal microbiota transplantation from young donor mice improves ovarian function in aged mice

doi: 10.1016/j.jgg.2022.05.006

a. State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu 211166, China;
b. Department of Pathogen Biology-Microbiology Division, State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu 211166, China;
c. Shanghai Personal Biotechnology, Shanghai 200231, China;
d. Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210003, China;
e. Institute of Pediatric Research, Children's Hospital of Soochow University, Suzhou, Jiangsu 215003, China;
f. Fertility Preservation Laboratory, Reproductive Medicine Center, Guangdong Second Provincial General Hospital, Guangzhou, Guangdong 510317, China

Funds:

This work was supported by the National Key Research and Development Program of China (2018YFC1003703-1) and the National Natural Science Foundation of China (81871628, 82172288, and 81902027).

Received Date: 2021-11-22
Accepted Date: 2022-05-19
Rev Recd Date: 2022-05-18
Publish Date: 2022-05-30

Abstract

Abstract

Advanced maternal age is characterized by declines in the quantity and quality of oocytes in the ovaries, and the aging process is accompanied by changes in gut microbiota composition. However, little is known about the relationship between gut microbiota and ovarian aging. By using fecal microbiota transplantation (FMT) to transplant material from young (5-week-old) into aged (42-week-old) mice, we find that the composition of gut microbiota in FMT-treated mice presents a “younger-like phenotype” and an increase of commensal bacteria, such as Bifidobacterium and Ruminococcaceae. Moreover, the FMT-treated mice show increased anti-inflammatory cytokine IL-4 and decreased pro-inflammatory cytokine IFN-γ. Fertility tests for assessing ovarian function reveal that the first litter size of female FMT-treated mice is significantly higher than that of the non-FMT group. Morphology analysis demonstrates a dramatic decrease in follicle atresia and apoptosis as well as an increase in cellular proliferation in the ovaries of the FMT-treated mice. Our results also show that FMT improves the immune microenvironment in aged ovaries, with decreased macrophages and macrophage-derived multinucleated giant cells (MNGCs). These results suggest that FMT from young donors could be a good choice for delaying ovarian aging.
- Ovarian aging,
- Gut microbiota,
- Inflammatory cytokines,
- Ovarian microenvironment

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References(47)

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