Diverse interactions of five core type III effectors from <i>Ralstonia solanacearum</i> with plants

Shen Cong; Jun-Zhou Li; Zheng-Zhong Xiong; Hai-Lei Wei

doi:10.1016/j.jgg.2022.04.018

Volume 50 Issue 5

May 2023

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Article Navigation > Journal of Genetics and Genomics > 2023 > 50(5): 341-352

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Diverse interactions of five core type III effectors from Ralstonia solanacearum with plants

doi: 10.1016/j.jgg.2022.04.018

Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Funds:

This work was supported by the National Key R&D Program of China (2019YFD1002000), the Science and Technology Programs of the Shandong Tobacco (KN273), and Zunyi Tobacco (2021XM03).

Received Date: 2022-01-30
Accepted Date: 2022-04-30
Rev Recd Date: 2022-04-29
Publish Date: 2022-05-18

Abstract

Abstract

Ralstonia solanacearum is a widespread plant bacterial pathogen that can launch a range of type III effectors (T3Es) to cause disease. In this study, we isolate a pathogenic R. solanacearum strain named P380 from tomato rhizosphere. Five out of 12 core T3Es of strain P380 are introduced into Pseudomonas syringae DC3000D36E separately to determine their functions in interacting with plants. DC3000D36E that harbors each effector suppresses FliC-triggered Pti5 and ACRE31 expression, ROS burst, and callose deposition. RipAE, RipU, and RipW elicit cell death as well as upregulate the MAPK cascades in Nicotiana benthamiana. The derivatives RipC1^ΔDXDX(T/V) and RipW^ΔDKXXQ but not RipAE^K310R fail to suppress ROS burst. Moreover, RipAE^K310R and RipW^ΔDKXXQ retain the cell death elicitation ability. RipAE and RipW are associated with salicylic acid and jasmonic acid pathways, respectively. RipAE and RipAQ significantly promote the propagation of DC3000D36E in plants. The five core T3Es localize in diverse subcellular organelles of nucleus, plasma membrane, endoplasmic reticulum, and Golgi network. The suppressor of G2 allele of Skp1 is required for RipAE but not RipU-triggered cell death in N. benthamiana. These results indicate that the core T3Es in R. solanacearum play diverse roles in plant-pathogen interactions.
- Ralstonia solanacearum,
- Type III secretion system,
- Effectors,
- PTI,
- ETI

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References(70)

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