OsLTP47 may function in a lipid transfer relay essential for pollen wall development in rice

Libin Chen; Chonghui Ji; Degui Zhou; Xin Gou; Jianian Tang; Yongjie Jiang; Jingluan Han; Yao-Guang Liu; Letian Chen; Yongyao Xie

doi:10.1016/j.jgg.2022.03.003

Volume 49 Issue 5

May 2022

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Article Navigation > Journal of Genetics and Genomics > 2022 > 49(5): 481-491

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OsLTP47 may function in a lipid transfer relay essential for pollen wall development in rice

doi: 10.1016/j.jgg.2022.03.003

a State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, China;
b Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, Guangdong 510642, China;
c Guangdong Key Laboratory of New Technology in Rice Breeding, Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong 510640, China

Funds:

This work was supported by grants from the Guangdong Natural Science Funds for Distinguished Young Scholars (2021B1515020089), the National Natural Science Foundation of China (32030080) and the Major Program of Guangdong Basic and Applied Research (2019B030302006).

Received Date: 2021-11-09
Accepted Date: 2022-03-05
Rev Recd Date: 2022-03-02
Publish Date: 2022-03-21

Abstract

Abstract

In plants, lipid transfer proteins (LTPs) transport pollen wall constituents from the tapetum to the exine, a process essential for pollen wall development. However, the functional cooperation of different LTPs in pollen wall development is not well understood. In this study, we have identified and characterized a grass-specific LTP gene, OsLTP47, an important regulator of pollen wall formation in rice (Oryza sativa). OsLTP47 encodes a membrane-localized LTP and in vitro lipid-binding assays confirms that OsLTP47 has lipid-binding activity. Dysfunction of OsLTP47 causes disordered lipid metabolism and defective pollen walls, leading to male sterility. Yeast two-hybrid and pull-down assays reveal that OsLTP47 physically interacts with another LTP, OsC6. These findings suggest that the plasma membrane-localized OsLTP47 may function as a mediator in a lipid transfer relay through association with cytosolic and/or locular OsC6 for pollen wall development and that various LTPs may function in a coordinated manner to transport lipid molecules during pollen wall development.
- Rice,
- Pollen wall,
- Lipid transfer protein,
- Male sterility

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References(41)

References

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