Polyamine oxidase 3 is involved in salt tolerance at the germination stage in rice

Guangyu Liu; Wanxia Jiang; Lei Tian; Yongcai Fu; Lubin Tan; Zuofeng Zhu; Chuanqing Sun; Fengxia Liu

doi:10.1016/j.jgg.2022.01.007

Volume 49 Issue 5

May 2022

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Article Navigation > Journal of Genetics and Genomics > 2022 > 49(5): 458-468

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Polyamine oxidase 3 is involved in salt tolerance at the germination stage in rice

doi: 10.1016/j.jgg.2022.01.007

a State Key Laboratory of Plant Physiology and Biochemistry, China Agricultural University, Beijing 100193, China;
b National Center for Evaluation of Agricultural Wild Plants (Rice), Beijing Key Laboratory of Crop Genetic Improvement, Laboratory of Crop Heterosis and Utilization, MOE, Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100193, China

Funds:

Grateful thanks to Prof. Yan Guo, College of Biology, China Agricultural University, who read the manuscript critically. This research was supported by self-regulated projects of the State Key Laboratory of Plant Physiology and Biochemistry and the National Natural Science Foundation of China (3137158).

Received Date: 2021-09-24
Accepted Date: 2022-01-18
Rev Recd Date: 2022-01-17
Publish Date: 2022-02-07

Abstract

Abstract

Soil salinity inhibits seed germination and reduces seedling survival rate, resulting in significant yield reductions in crops. Here, we report the identification of a polyamine oxidase, OsPAO3, conferring salt tolerance at the germination stage in rice (Oryza sativa L.), through map-based cloning approach. OsPAO3 is up-regulated under salt stress at the germination stage and highly expressed in various organs. Overexpression of OsPAO3 increases activity of polyamine oxidases, enhancing the polyamine content in seed coleoptiles. Increased polyamine may lead to the enhance of the activity of ROS-scavenging enzymes to eliminate over-accumulated H₂O₂ and to reduce Na⁺ content in seed coleoptiles to maintain ion homeostasis and weaken Na⁺ damage. These changes resulted in stronger salt tolerance at the germination stage in rice. Our findings not only provide a unique gene for breeding new salt-tolerant rice cultivars but also help to elucidate the mechanism of salt tolerance in rice.
- Polyamine oxidase,
- OsPAO3,
- Salt tolerance,
- Germination stage,
- Rice

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References

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