The sex determination gene <i>doublesex</i> regulates expression and secretion of the basement membrane protein Collagen IV

Qionglin Peng; Jiangtao Chen; Rong Wang; Huan Zhu; Caihong Han; Xiaoxiao Ji; Yufeng Pan

doi:10.1016/j.jgg.2021.12.010

Volume 49 Issue 7

Jul. 2022

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The sex determination gene doublesex regulates expression and secretion of the basement membrane protein Collagen IV

doi: 10.1016/j.jgg.2021.12.010

a The Key Laboratory of Developmental Genes and Human Disease, School of Life Science and Technology, Southeast University, Nanjing, Jiangsu 210096, China;
b Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu 226019, China

Funds:

We thank the Bloomington Drosophila Stock Center, Tsinghua Fly Center and Dr. José Carlos Pastor-Pareja in Tsinghua University for fly stocks. This work was supported by grants from National Key R&

D Program of China (2019YFA0802400), the National Natural Science Foundation of China (31970943 and 31700905), and the Jiangsu Innovation and Entrepreneurship Team Program.

Received Date: 2021-09-06
Accepted Date: 2021-12-24
Rev Recd Date: 2021-12-19
Publish Date: 2022-01-10

Abstract

Abstract

The highly conserved doublesex (dsx) and doublesex/mab-3 related (Dmrt) genes control sexually dimorphic traits across animals. The dsx gene encodes sex-specific transcription factors, Dsx^M in males and Dsx^F in females, which function differentially and often oppositely to establish sexual dimorphism. Here, we report that mutations in dsx, or overexpression of dsx, result in abnormal distribution of the basement membrane (BM) protein Collagen IV in the fat body. We find that Dsx isoforms regulate the expression of Collagen IV in the fat body and its secretion into the BM of other tissues. We identify the procollagen lysyl hydroxylase (dPlod) gene, which is involved in the biosynthesis of Collagen IV, as a direct target of Dsx. We further show that Dsx regulates Collagen IV through dPlod-dependent and independent pathways. These findings reveal how Dsx isoforms function in the secretory fat body to regulate Collagen IV and remotely establish sexual dimorphism.
- Drosophila,
- Sexual dimorphism,
- doublesex,
- Dmrt genes,
- Collagen IV,
- dPlod,
- Fat body

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References(41)

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