Identifying a melanogenesis-related candidate gene by a high-quality genome assembly and population diversity analysis in Hypsizygus marmoreus

Gang Wang; Lianfu Chen; Weiqi Tang; Yuanyuan Wang; Qing Zhang; Hongbo Wang; Xuan Zhou; Haofeng Wu; Lin Guo; Meijie Dou; Lei Liu; Baiyu Wang; Jingxian Lin; Baogui Xie; Zhengchao Wang; ZhongJian Liu; Ray Ming; Jisen Zhang

doi:10.1016/j.jgg.2021.01.002

Volume 48 Issue 1

Jan. 2021

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Article Navigation > Journal of Genetics and Genomics > 2021 > 48(1): 75-87

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Identifying a melanogenesis-related candidate gene by a high-quality genome assembly and population diversity analysis in Hypsizygus marmoreus

doi: 10.1016/j.jgg.2021.01.002

a
Center for Genomics and Biotechnology, Haixia Institute of Science and Technology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
b
School of Geographical Science, Nantong University, Nantong 226001, China
c
South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
d
Institute of Oceanography, Marine Biotechnology Center, Minjiang University, Fuzhou 350108, China
e
College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
f
Provincial Key Laboratory for Developmental Biology and Neurosciences, College of Life Sciences, Fujian Normal University, Fuzhou 350007, China
g
Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
h
Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

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Corresponding author: E-mail address: zjisen@126.com (Jisen Zhang)
Publish Date: 2021-01-20

Abstract

Abstract

Hypsizygus marmoreus is one of the most important edible fungi in Basidiomycete division and includes white and gray strains. However, very limited knowledge is known about the genomic structures and the genetic basis for the white/gray diversity of this mushroom. Here, we report the near-complete high-quality H. marmoreus genome at the chromosomal level. Comparative genomics analysis indicates that chromosome structures were relatively conserved, and variations in collinearity and chromosome number were mainly attributed by chromosome split/fusion events inAragicales, whereas the fungi genome experienced many genomic chromosome fracture, fusion, and genomic replication events after the split of Aragicales from Basidiomycetes. Resequencing of 57 strains allows us to classify the population into four major groups and associate genetic variations with morphological features, indicating that white strains were not originated independently. We further generated genetic populations and identified a cytochrome P450 as the candidate causal gene for the melanogenesis in H. marmoreus based on bulked segregant analysis (BSA) and comparative transcriptome analysis. The high-quality H. marmoreus genome and diversity data compiled in this study provide new knowledge and resources for the molecular breeding of H. marmoreus as well as the evolution of Basidiomycete.
- Bulked segregant analysis (BSA),
- Melanogenesis

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References(76)

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