Single-cell RNA sequencing identifies novel cell types in Drosophila blood

Yulong Fu; Xiaohu Huang; Peng Zhang; Joyce van de Leemput; Zhe Han

doi:10.1016/j.jgg.2020.02.004

Volume 47 Issue 4

Apr. 2020

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Article Navigation > Journal of Genetics and Genomics > 2020 > 47(4): 175-186

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Single-cell RNA sequencing identifies novel cell types in Drosophila blood

doi: 10.1016/j.jgg.2020.02.004

Yulong Fu^{a, 1},
Xiaohu Huang^{a, c, 1},
Peng Zhang^b,
Joyce van de Leemput^{a, c},
Zhe Han^{a, c}

a
Center for Precision Disease Modeling, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, 21201, USA
b
Divisions of Immunotherapy, University of Maryland School of Medicine, Baltimore, MD, 21201, USA
c
Division of Endocrinology, Diabetes and Nutrition, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, 21201, USA

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Corresponding author: E-mail address: zhan@som.umaryland.edu (Zhe Han)
Publish Date: 2020-04-25

Abstract

Abstract

Drosophila has been extensively used to model the human blood-immune system, as both systems share many developmental and immune response mechanisms. However, while many human blood cell types have been identified, only three were found in flies: plasmatocytes, crystal cells and lamellocytes. To better understand the complexity of fly blood system, we used single-cell RNA sequencing technology to generate comprehensive gene expression profiles for Drosophila circulating blood cells. In addition to the known cell types, we identified two new Drosophila blood cell types: thanacytes and primocytes. Thanacytes, which express many stimulus response genes, are involved in distinct responses to different types of bacteria. Primocytes, which express cell fate commitment and signaling genes, appear to be involved in keeping stem cells in the circulating blood. Furthermore, our data revealed four novel plasmatocyte subtypes (Ppn⁺, CAH7⁺, Lsp⁺ and reservoir plasmatocytes), each with unique molecular identities and distinct predicted functions. We also identified cross-species markers from Drosophila hemocytes to human blood cells. Our analysis unveiled a more complex Drosophila blood system and broadened the scope of using Drosophila to model human blood system in development and disease.
- Blood,
- Single-cell RNA-seq,
- Thanacyte,
- Primocyte,
- Plasmatocyte

These authors contributed equally.

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References(36)

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