Advances in detecting and reducing off-target effects generated by CRISPR-mediated genome editing

Jinjing Li; Shunyan Hong; Wanjin Chen; Erwei Zuo; Hui Yang

doi:10.1016/j.jgg.2019.11.002

Volume 46 Issue 11

Nov. 2019

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Article Navigation > Journal of Genetics and Genomics > 2019 > 46(11): 513-521

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Advances in detecting and reducing off-target effects generated by CRISPR-mediated genome editing

doi: 10.1016/j.jgg.2019.11.002

Jinjing Li^{a, b, #},
Shunyan Hong^{a, b, #},
Wanjin Chen^{a, b},
Erwei Zuo^c,
Hui Yang^d

a
Department of Neurology and Institute of Neurology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, China
b
Fujian Key Laboratory of Molecular Neurology, Fujian Medical University, Fuzhou, 350005, China
c
Lingnan Guangdong Laboratory of Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518124, China
d
Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Research Center for Brain Science and Brain-Inspired Intelligence, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China

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Corresponding author: E-mail address: wanjinchen75@fjmu.edu.cn (Wanjin Chen); E-mail address: erweizuo@163.com (Erwei Zuo); E-mail address: huiyang@ion.ac.cn (Hui Yang)
Received Date: 2019-10-07
Accepted Date: 2019-11-14
Rev Recd Date: 2019-11-05

Available Online: 2019-11-22

Publish Date: 2019-11-20

Abstract

Abstract

CRISPR-mediated genome editing is a revolutionary technology for genome manipulation that uses the CRISPR-Cas systems and base editors. Currently, poor efficiency and off-target problems have impeded the application of CRISPR systems. The on-target efficiency has been improved in several advanced versions of CRISPR systems, whereas the off-target detection still remains a key challenge. Here, we outline the different versions of CRISPR systems and off-target detection strategies, discuss the merits and limitations of off-target detection methods, and provide potential implications for further gene editing research.
- Gene editing,
- CRISPR-Cas9,
- Base editors,
- Off-target

These authors contributed equally.

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References(90)

References

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