Temperature-sensitive cytoophidium assembly in Schizosaccharomyces pombe

Jing Zhang; Ji-Long Liu

doi:10.1016/j.jgg.2019.09.002

Volume 46 Issue 9

Sep. 2019

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Article Navigation > Journal of Genetics and Genomics > 2019 > 46(9): 423-432

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Temperature-sensitive cytoophidium assembly in Schizosaccharomyces pombe

doi: 10.1016/j.jgg.2019.09.002

Jing Zhang^a,
Ji-Long Liu^{a, b}

a
MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, OX1 3PT, United Kingdom
b
School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China

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Corresponding author: E-mail address: jilong.liu@dpag.ox.ac.uk (Ji-Long Liu)
Received Date: 2019-04-19
Accepted Date: 2019-09-09
Rev Recd Date: 2019-08-29

Available Online: 2019-09-24

Publish Date: 2019-09-20

Abstract

Abstract

The metabolic enzyme CTP synthase (CTPS) is able to compartmentalize into filaments, termed cytoophidia, in a variety of organisms including bacteria, budding yeast, fission yeast, fruit flies and mammals. A previous study in budding yeast shows that the filament-forming process of CTPS is not sensitive to temperature shift. Here we study CTPS filamentation in the fission yeast Schizosaccharomyces pombe. To our surprise, we find that both the length and the occurrence of cytoophidia in S. pombe decrease upon cold shock or heat shock. The temperature-dependent changes of cytoophidia are fast and reversible. Taking advantage of yeast genetics, we demonstrate that heat-shock proteins are required for cytoophidium assembly in S. pombe. Temperature sensitivity of cytoophidia makes S. pombe an attractive model system for future investigations of this novel membraneless organelle.
- CTP synthase,
- Cytoophidium,
- Heat-shock protein,
- Nucleoside/nucleotide metabolism,
- Cell biology,
- Yeast genetics,
- Cell compartmentalization

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References(30)

References

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