Perspectives on gene expression regulation techniques in Drosophila

Rong-Gang Xu; Xia Wang; Da Shen; Jin Sun; Huan-Huan Qiao; Fang Wang; Lu-Ping Liu; Jian-Quan Ni

doi:10.1016/j.jgg.2019.03.006

Volume 46 Issue 4

Apr. 2019

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Perspectives on gene expression regulation techniques in Drosophila

doi: 10.1016/j.jgg.2019.03.006

Rong-Gang Xu^a,
Xia Wang^a,
Da Shen^a,
Jin Sun^{a, b},
Huan-Huan Qiao^a,
Fang Wang^a,
Lu-Ping Liu^{a, c, d},
Jian-Quan Ni^{a, e}

a
Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing, 100084, China
b
Tsinghua University-Peking University Joint Center for Life Sciences, Beijing, 100084, China
c
Department of Genetics, Harvard Medical School, Boston, MA, 02115, USA
d
Howard Hughes Medical Institute, Boston, MA, 02115, USA
e
Tsingdao Advanced Research Institute, Tongji University, Qingdao, 266000, China

More Information

Corresponding author: E-mail address: nijq@mail.tsinghua.edu.cn (Jian-Quan Ni)
Received Date: 2018-11-19
Accepted Date: 2019-03-12
Rev Recd Date: 2019-02-27

Available Online: 2019-04-17

Publish Date: 2019-04-20

Abstract

Abstract

Gene expression regulation, including loss-of-function and gain-of-function assays, is a powerful method to study developmental and disease mechanisms. Drosophila melanogaster is an ideal model system particularly well-equipped with many genetic tools. In this review, we describe and discuss the gene expression regulation techniques recently developed and their applications, including the CRISPR/Cas9-triggered heritable mutation system, CRISPR/dCas9-based transcriptional activation (CRISPRa) system, and CRISPR/dCas9-based transcriptional repression (CRISPRi) system, as well as the next-generation transgenic RNAi system. The main purpose of this review is to provide the fly research community with an updated summary of newly developed gene expression regulation techniques and help the community to select appropriate methods and optimize the research strategy.
- Genome editing system,
- CRISPRa,
- CRISPRi,
- Transgenic RNAi system

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References(113)

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