Arabidopsis AGC protein kinases IREH1 and IRE3 control root skewing

Xiaozhen Yue; Zhiai Guo; Teng Shi; Lizhen Song; Youfa Cheng

doi:10.1016/j.jgg.2019.02.007

Volume 46 Issue 5

May 2019

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Arabidopsis AGC protein kinases IREH1 and IRE3 control root skewing

doi: 10.1016/j.jgg.2019.02.007

Xiaozhen Yue^{a, b},
Zhiai Guo^a,
Teng Shi^{a, b},
Lizhen Song^a,
Youfa Cheng^{a, b}

a
Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
b
University of Chinese Academy of Sciences, Beijing 100049, China

More Information

Corresponding author: E-mail address: yfcheng@ibcas.ac.cn (Youfa Cheng)
Received Date: 2018-11-26
Accepted Date: 2019-02-25
Rev Recd Date: 2019-02-04

Available Online: 2019-03-22

Publish Date: 2019-05-20

Abstract

Abstract

AGC protein kinases play important roles in plant growth and development. Several AGC kinases in Arabidopsis have been functionally characterized. However, the “AGC Other” subfamily, including IRE, IREH1, IRE3 and IRE4, has not been well understood. Here, we reported that ireh1 mutants displayed a root skewing phenotype, which can be enhanced by ire3 mutation. IREH1 and IRE3 were expressed in roots, consistent with their function in controlling root skewing. The fluorescence intensities of the microtubule marker KNpro:EGFP-MBD were decreased in ireh1, ire3 and ireh1 ire3 mutants compared to wild type. The microtubule arrangements in ireh1 and ireh1 ire3 mutants were also altered. IREH1 physically interacted with IRE3 in vitro and in planta. Thus, our findings demonstrate that IREH1 and IRE3 protein kinases play important roles in controlling root skewing, and maintaining microtubule network in Arabidopsis.
- AGC kinases,
- IREH1,
- IRE3,
- Root skewing,
- Microtubule

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References(40)

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