Whole-brain patterns of the presynaptic inputs and axonal projections of BDNF neurons in the paraventricular nucleus

Fang Luo; Yuling Mu; Cuicui Gao; Yan Xiao; Qian Zhou; Yiqing Yang; Xinyan Ni; Wei L. Shen; Jiajun Yang

doi:10.1016/j.jgg.2018.11.004

Volume 46 Issue 1

Jan. 2019

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Whole-brain patterns of the presynaptic inputs and axonal projections of BDNF neurons in the paraventricular nucleus

doi: 10.1016/j.jgg.2018.11.004

Fang Luo^{a, #},
Yuling Mu^{a, #},
Cuicui Gao^{a, b, c, #},
Yan Xiao^{d, e},
Qian Zhou^{a, b, c},
Yiqing Yang^a,
Xinyan Ni^a,
Wei L. Shen^a,
Jiajun Yang^{d, e}

a
Undergraduate Program and School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China
b
Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China
c
University of Chinese Academy of Sciences, Beijing, 100049, China
d
Department of Neurology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China
e
Department of Neurology, Shanghai Sixth People's Hospital East Affiliated to Shanghai University of Medicine & Health Science, Shanghai, 201306, China

More Information

Corresponding author: E-mail address: shenwei@shanghaitech.edu.cn (Wei L. Shen); E-mail address: sd_yangjj@sumhs.edu.cn (Jiajun Yang)
Received Date: 2018-11-03
Accepted Date: 2018-11-25
Rev Recd Date: 2018-11-21

Available Online: 2019-01-26

Publish Date: 2019-01-20

Abstract

Abstract

Brain-derived neurotrophic factor (BDNF) plays a crucial role in human obesity. Yet, the neural circuitry supporting the BDNF-mediated control of energy homeostasis remains largely undefined. To map key regions that might provide inputs to or receive inputs from the paraventricular nucleus (PVN) BDNF neurons, a key type of cells in regulating feeding and thermogenesis, we used rabies virus-based transsynaptic labeling and adeno-associated virus based anterograde tracing techniques to reveal their whole-brain distributions. We found that dozens of brain regions provide dense inputs to or receive dense inputs from PVN BDNF neurons, including several known weight control regions and several novel regions that might be functionally important for the BDNF-mediated regulation of energy homeostasis. Interestingly, several regions show very dense reciprocal connections with PVN BDNF neurons, including the lateral septum, the preoptic area, the ventromedial hypothalamic nucleus, the paraventricular thalamic nucleus, the zona incerta, the lateral parabrachial nucleus, the subiculum, the raphe magnus nucleus, and the raphe pallidus nucleus. These strong anatomical connections might be indicative of important functional connections. Therefore, we provide an outline of potential neural circuitry mediated by PVN BDNF neurons, which might be helpful to resolve the complex obesity network.
- BDNF,
- Transsynaptic tracing,
- Obesity,
- Feeding,
- Thermogenesis

These authors contributed equally.

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References(58)

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