prpf4 is essential for cell survival and posterior lateral line primordium migration in zebrafish

Yixia Wang; Yanchao Han; Pengfei Xu; Shihui Ding; Guangyuan Li; Hongbin Jin; Yaping Meng; Anming Meng; Shunji Jia

doi:10.1016/j.jgg.2018.05.008

Volume 45 Issue 8

Aug. 2018

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prpf4 is essential for cell survival and posterior lateral line primordium migration in zebrafish

doi: 10.1016/j.jgg.2018.05.008

State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China

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Corresponding author: E-mail address: jiasj@mail.tsinghua.edu.cn (Shunji Jia)
Received Date: 2018-01-24
Accepted Date: 2018-05-17
Rev Recd Date: 2018-04-20

Available Online: 2018-08-07

Publish Date: 2018-08-20

Abstract

Abstract

Prpf4 (pre-mRNA processing factor 4), a key component of spliceosome, plays critical roles in pre-mRNA splicing and its mutations result in retinitis pigmentosa due to photoreceptor defects. In this study, we characterized a zebrafish prpf4 mutant harboring a Tol2 transposon-based gene trap cassette in the third intron of the prpf4 gene. Cells in the brain and spinal cord gradually undergo p53-dependent apoptosis after 28 hpf in prpf4 mutants, suggesting that a widespread function of prpf4 in neural cell survival. In addition, prpf4 is essential for survival of posterior lateral line primordial (pLLP) cells. prpf4 deficiency perturbs Fgf, Wnt/β-catenin and chemokine signaling pathways and impairs pLLP migration. RNA-Seq analysis suggests that prpf4 deficiency may impair spliceosome assembly, leading to compensatory upregulation of core spliceosomal genes and alteration of pre-mRNA splicing. Taken together, our studies uncover an essential role of prpf4 in pre-mRNA splicing, cell survival and pLLP migration.
- Splicing,
- Apoptosis,
- pLLP,
- Zebrafish

These authors contributed equally to this work.

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References(41)

References

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