Caudal dorsal artery generates hematopoietic stem and progenitor cells via the endothelial-to-hematopoietic transition in zebrafish

Yandong Zhan; Youkui Huang; Jingying Chen; Zigang Cao; Jianbo He; Jingjing Zhang; Honghui Huang; Hua Ruan; Lingfei Luo; Li Li

doi:10.1016/j.jgg.2018.02.010

Volume 45 Issue 6

Jun. 2018

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Article Navigation > Journal of Genetics and Genomics > 2018 > 45(6): 315-324

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Caudal dorsal artery generates hematopoietic stem and progenitor cells via the endothelial-to-hematopoietic transition in zebrafish

doi: 10.1016/j.jgg.2018.02.010

a
The State Key Laboratory Breeding Base of Bioresources and Eco-environments, Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Laboratory of Molecular Developmental Biology, School of Life Sciences, Southwest University, Chongqing 400715, China
b
Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China

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Corresponding author: E-mail address: lluo@swu.edu.cn (Lingfei Luo); E-mail address: alisir@swu.edu.cn (Li Li)
Received Date: 2017-09-05
Accepted Date: 2018-02-11
Rev Recd Date: 2017-12-25

Available Online: 2018-06-08

Publish Date: 2018-06-20

Abstract

Abstract

Zebrafish hematopoietic stem and progenitor cells (HSPCs) originate from the hemogenic endothelium of the ventral wall of the dorsal aorta (DA) through the endothelial-to-hematopoietic transition (EHT) from approximately 30 to 60 hours post fertilization (hpf). However, whether other artery sites can generate HSPCs de novo remains unclear. In this study, using live imaging and lineage tracing, we found that the caudal dorsal artery (CDA) in the caudal hematopoietic tissue directly gave rise to HSPCs through EHT. This process initiated from approximately 60 hpf and terminated at approximately 156 hpf. Compared with that in the DA, fewer EHT events were observed in the CDA. The EHT events in the DA and CDA were similarly regulated by Runx1 but differentially influenced by blood flow (i.e., the EHT frequency in CDA was affected to a lesser extent when circulation was compromised in thetnnt2a mutant). Therefore, the whole artery, including both DA and CDA, was endowed with the ability to produce HSPCs during a much longer time period. Coincidently, the lineage tracing results indicated that adult hematopoietic cells originated from the embryonic endothelium, and those produced later preferentially colonized the adult thymus. Collectively, our study revealed that the CDA serves as an additional source of hematopoiesis, and it shows similar but not identical properties with the DA.
- Caudal dorsal artery,
- Endothelial-to-hematopoietic transition,
- Circulation,
- Zebrafish

These authors contributed equally to this work.

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