Ribosome biogenesis protein Urb1 acts downstream of mTOR complex 1 to modulate digestive organ development in zebrafish

Jia He; Yun Yang; Junren Zhang; Jinzi Chen; Xiangyong Wei; Jianbo He; Lingfei Luo

doi:10.1016/j.jgg.2017.09.013

Volume 44 Issue 12

Dec. 2017

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Ribosome biogenesis protein Urb1 acts downstream of mTOR complex 1 to modulate digestive organ development in zebrafish

doi: 10.1016/j.jgg.2017.09.013

Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Laboratory of Molecular Developmental Biology, School of Life Sciences, Southwest University, Chongqing 400715, China

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Corresponding author: E-mail address: lluo@swu.edu.cn (Lingfei Luo)
Received Date: 2017-05-27
Accepted Date: 2017-09-28
Rev Recd Date: 2017-09-27

Available Online: 2017-11-29

Publish Date: 2017-12-20

Abstract

Abstract

Ribosome biogenesis is essential for the cell growth and division. Disruptions in ribosome biogenesis result in developmental defects and a group of diseases, known as ribosomopathies. Here, we report a mutation in zebrafish urb1, which encodes an essential ribosome biogenesis protein. Theurb1 mutant exhibits hypoplastic digestive organs, which is caused by impaired cell proliferation with the differentiation of digestive organ progenitors unaffected. Knockdown of mtor or raptor leads to similar hypoplastic phenotypes and reduced expression of urb1 in the digestive organs. Overexpression of Urb1 results in overgrowth of digestive organs, and can efficiently rescue the hypoplastic liver and pancreas in the mtor and raptor morphants. Reduced syntheses of free ribosomal subunits and impaired assembly of polysomes are observed in the urb1 mutant as well as in the mtor and raptor morphants, which can be rescued by the Urb1 overexpression. These data demonstrate that Urb1 plays an important role in governing ribosome biogenesis and protein synthesis downstream of mammalian/mechanistic target of rapamycin complex 1 (mTORC1), thus regulating the development of digestive organs. Our study indicates the requirement of hyperactive protein synthesis for the digestive organ development.
- Ribosome biogenesis protein,
- Urb1,
- Digestive organ development,
- mTORC1,
- Zebrafish

These authors contributed equally to this work.

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References(44)

References

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