Key elements for designing and performing a CRISPR/Cas9-based genetic screen

Wanjing Shang; Fei Wang; Gaofeng Fan; Haopeng Wang

doi:10.1016/j.jgg.2017.09.005

Volume 44 Issue 9

Sep. 2017

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Article Navigation > Journal of Genetics and Genomics > 2017 > 44(9): 439-449

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Key elements for designing and performing a CRISPR/Cas9-based genetic screen

doi: 10.1016/j.jgg.2017.09.005

Wanjing Shang^{a, b, c, #},
Fei Wang^{a, b, c, #},
Gaofeng Fan^a,
Haopeng Wang^a

a
School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China
b
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
c
University of Chinese Academy of Sciences, Beijing 100049, China

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Corresponding author: E-mail address: fangf@shanghaitech.edu.cn (Gaofeng Fan); E-mail address: wanghp@shanghaitech.edu.cn (Haopeng Wang)
Received Date: 2017-06-01
Accepted Date: 2017-09-04
Rev Recd Date: 2017-07-09

Available Online: 2017-09-22

Publish Date: 2017-09-20

Abstract

Abstract

Reverse genetic screens are invaluable for uncovering gene functions, but are traditionally hampered by some technical limitations. Over the past few years, since the advent of the revolutionary CRISPR/Cas9 technology, its power in genome editing has been harnessed to overcome the traditional limitations in reverse genetic screens, with successes in various biological contexts. Here, we outline these CRISPR/Cas9-based screens, provide guidance on the design of effective screens and discuss the potential future directions of development of this field.
- CRISPR,
- Cas9,
- Genetic screen

These authors contributed equally to this work.

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References(95)

References

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