Significant differences of function and expression of microRNAs between ground state and serum-cultured pluripotent stem cells

Ying Yan; Xi Yang; Ting-Ting Li; Kai-Li Gu; Jing Hao; Qiang Zhang; Yangming Wang

doi:10.1016/j.jgg.2017.01.005

Volume 44 Issue 4

Apr. 2017

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Significant differences of function and expression of microRNAs between ground state and serum-cultured pluripotent stem cells

doi: 10.1016/j.jgg.2017.01.005

a
Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Institute of Molecular Medicine, Peking University, Beijing 100871, China
b
Department of Biomedical Informatics, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100871, China

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Corresponding author: E-mail address: yangming.wang@pku.edu.cn (Yangming Wang)
Received Date: 2016-11-01
Accepted Date: 2017-01-16
Rev Recd Date: 2017-01-16

Available Online: 2017-02-22

Publish Date: 2017-04-20

Abstract

Abstract

Serum- and 2i-cultured embryonic stem cells (ESCs) show different epigenetic landscapes and transcriptomic profiles. The difference in the function and expression of microRNAs (miRNAs) between these two states remains unclear. Here, we showed that 2i- and serum-cultured ESCs exhibited distinctive miRNA expression profiles with >100 miRNAs differentially expressed, and the expression changes were largely due to transcriptional regulation. We further characterized the function of miRNAs differentially expressed under two conditions and found that ESCs exhibited higher degree of dependency on miRNAs for rapid proliferation; since or but not wild-type ESCs showed slower growth rate and more accumulation in the G1 phase under 2i than serum condition. More interestingly, introduction of various self-renewal-silencing miRNAs in wild-type or ESCs failed to silence the self-renewal in 2i medium, but regained the ability to silence the self-renewal upon the addition of serum. Our findings reveal significant differences in the expression and function of miRNAs between serum- and 2i-cultured ESCs.
- Embryonic stem cells,
- microRNAs,
- Ground pluripotency state,
- Self-renewal

These authors contributed equally to this work.

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References

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