The OsAMT1.1 gene functions in ammonium uptake and ammonium–potassium homeostasis over low and high ammonium concentration ranges

Chang Li; Zhong Tang; Jia Wei; Hongye Qu; Yanjie Xie; Guohua Xu

doi:10.1016/j.jgg.2016.11.001

Volume 43 Issue 11

Nov. 2016

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Article Navigation > Journal of Genetics and Genomics > 2016 > 43(11): 639-649

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The OsAMT1.1 gene functions in ammonium uptake and ammonium–potassium homeostasis over low and high ammonium concentration ranges

doi: 10.1016/j.jgg.2016.11.001

a
State Key Laboratory of Crop Genetics and Germplasm Enhancement and MOA Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China
b
Laboratory Center of Life Sciences, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China

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Corresponding author: E-mail address: ghxu@njau.edu.cn (Guohua Xu)
Received Date: 2016-06-29
Accepted Date: 2016-11-03
Rev Recd Date: 2016-09-16

Available Online: 2016-11-05

Publish Date: 2016-11-20

Abstract

Abstract

Rice (Oryza sativa) grown in paddy fields is an ammonium (NH₄⁺)-preferring crop; however, its AMT-type NH₄⁺ transporters that mediate root N acquisition have not been well characterized yet. In this study, we analyzed the expression pattern and physiological function of the OsAMT1.1 gene of the AMT1 subfamily in rice. OsAMT1.1 is located in the plasma membrane and is mainly expressed in the root epidermis, stele and mesophyll cells. Disruption of the OsAMT1.1 gene decreased the uptake of NH₄⁺, and the growth of roots and shoots under both low NH₄⁺ and high NH₄⁺ conditions. OsAMT1.1 contributed to the short-term (5 min) ¹⁵NH₄⁺ influx rate by approximately one-quarter, irrespective of the NH₄⁺ concentration. Knockout of OsAMT1.1 significantly decreased the total N transport from roots to shoots under low NH₄⁺ conditions. Moreover, compared with the wild type, the osamt1.1 mutant showed an increase in the potassium (K) absorption rate under high NH₄⁺ conditions and a decrease under low NH₄⁺ conditions. The mutants contained a significantly high concentration of K in both the roots and shoots at a limited K (0.1 mmol/L) supply when NH₄⁺ was replete. Taken together, the results indicated that OsAMT1.1 significantly contributes to the NH₄⁺ uptake under both low and high NH₄⁺ conditions and plays an important role in N–K homeostasis in rice.
- Ammonium,
- Ammonium transporter,
- Interaction,
- Potassium,
- Rice

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References(55)

References

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