Cebpα is essential for the embryonic myeloid progenitor and neutrophil maintenance in zebrafish

Yimei Dai; Lu Zhu; Zhibin Huang; Minyu Zhou; Wan Jin; Wei Liu; Mengchang Xu; Tao Yu; Yiyue Zhang; Zilong Wen; Wangjun Liao; Wenqing Zhang

doi:10.1016/j.jgg.2016.09.001

Volume 43 Issue 10

Oct. 2016

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Cebpα is essential for the embryonic myeloid progenitor and neutrophil maintenance in zebrafish

doi: 10.1016/j.jgg.2016.09.001

Yimei Dai^{a, #},
Lu Zhu^{a, #},
Zhibin Huang^{b, #},
Minyu Zhou^c,
Wan Jin^a,
Wei Liu^b,
Mengchang Xu^b,
Tao Yu^a,
Yiyue Zhang^b,
Zilong Wen^{a, b},
Wangjun Liao^c,
Wenqing Zhang^b

a
Division of Life Science, State Key Laboratory of Molecular Neuroscience, and Center of Systems Biology and Human Health, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
b
Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes, Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China
c
Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China

More Information

Corresponding author: E-mail address: zilong@ust.hk (Zilong Wen); E-mail address: nfyyliaowangjun@163.com (Wangjun Liao); E-mail address: zzwwqq@smu.edu.cn (Wenqing Zhang)
Received Date: 2016-04-22
Accepted Date: 2016-09-01
Rev Recd Date: 2016-08-29

Available Online: 2016-09-03

Publish Date: 2016-10-20

Abstract

Abstract

In vertebrates, myeloid cells arise from multiple waves of development: the first or embryonic wave of myelopoiesis initiates early from non-hematopoietic stem cell (HSC) precursors and gives rise to myeloid cells transiently during early development; whereas the second or adult wave of myelopoiesis emerges later from HSCs and produces myeloid cells continually during fetal and adult life. In the past decades, a great deal has been learnt about the development of myeloid cells from adult myelopoiesis, yet the genetic network governing embryonic myelopoiesis remains poorly defined. In this report, we present an in vivo study to delineate the role of Cebpα during zebrafish embryonic myelopoiesis. We show that embryonic myelopoiesis in cebpα-deficient zebrafish mutants initiates properly but fails to produce macrophages and neutrophils. The lack of macrophages and neutrophils in the mutants is largely attributed to the cell cycle arrest of embryonic myeloid progenitors, resulting in the impairment of their maintenance and subsequent differentiation. We further show that Cebpα, perhaps acting cooperatively with Runx1, plays a critical role in embryonic neutrophil maintenance. Our findings reveal a new role of Cebpα in embryonic myelopoiesis.
- Cebpα,
- Embryonic myelopoiesis,
- Development,
- Zebrafish

These authors contributed equally to this work.

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References(48)

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