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Volume 43 Issue 5
May  2016
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Article Navigation >  Journal of Genetics and Genomics  > 2016  >  43(5): 251-262

Targeted Gene Manipulation in Plants Using the CRISPR/Cas Technology

doi: 10.1016/j.jgg.2016.03.001
  •  

    State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China

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  • Corresponding author: E-mail address: lijfeng3@mail.sysu.edu.cn (Jian-Feng Li)
  • Received Date: 2016-01-03
  • Accepted Date: 2016-03-04
  • Rev Recd Date: 2016-03-02
    • Available Online: 2016-03-11
  • Publish Date: 2016-05-20
    Abstract
  • The CRISPR/Cas technology is emerging as a revolutionary genome editing tool in diverse organisms including plants, and has quickly evolved into a suite of versatile tools for sequence-specific gene manipulations beyond genome editing. Here, we review the most recent applications of the CRISPR/Cas toolkit in plants and also discuss key factors for improving CRISPR/Cas performance and strategies for reducing the off-target effects. Novel technical breakthroughs in mammalian research regarding the CRISPR/Cas toolkit will also be incorporated into this review in hope to stimulate prospective users from the plant research community to fully explore the potential of these technologies.
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