Enhanced Expression of miR-425 Promotes Esophageal Squamous Cell Carcinoma Tumorigenesis by Targeting SMAD2

Lingyan Liu; Zitong Zhao; Wei Zhou; Xinyi Fan; Qimin Zhan; Yongmei Song

doi:10.1016/j.jgg.2015.09.010

Volume 42 Issue 11

Nov. 2015

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Enhanced Expression of miR-425 Promotes Esophageal Squamous Cell Carcinoma Tumorigenesis by Targeting SMAD2

doi: 10.1016/j.jgg.2015.09.010

State Key Laboratory of Molecular Oncology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China

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Corresponding author: E-mail address: symlh2006@163.com (Yongmei Song)
Received Date: 2015-03-11
Accepted Date: 2015-09-28
Rev Recd Date: 2015-09-21

Available Online: 2015-10-09

Publish Date: 2015-11-20

Abstract

Abstract

Esophageal squamous cell carcinoma (ESCC) is one of the most common and deadly cancers in the world. Currently, clinical therapy of ESCC remains limited and the five-year survival rate is poor. The function of miR-425 has been reported in multiple human cancers. However, the tumorigenic role and clinical significance of miR-425 in ESCC remains unclear. We found that enhanced expression of miR-425 in ESCC cell lines not only promoted cell proliferation and colony formation, but also increased cellular metastasis. Furthermore, we revealed the mechanism that miR-425 inhibited the expression of SMAD2 by targeting the second binding site in the 3′-untranslated region (3′-UTR) in ESCC. This mode of action influenced not only SMAD2 mRNA expression but also protein expression. In addition, we detected the expression of miR-425 in ESCC tissues and plasma. Moreover, we analyzed the relationship between miR-425 expression and SMAD2 mRNA expression. We found that miR-425 was overexpressed in ESCC tissues and the plasma relative to adjacent normal tissues and plasma of healthy individuals. Furthermore, there was a negative correlation between miR-425 expression and SMAD2. Taken together, our results show that miR-425 functions as an oncogene by targeting the 3′-UTR of SMAD2 and indicate the potential utility of plasma miR-425 as a novel biomarker for ESCC diagnosis.
- Esophageal squamous cell carcinoma,
- miR-425,
- Plasma miRNA,
- SMAD2

These authors contributed equally to this work.

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References

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