The RING Finger Protein NtRCP1 Is Involved in the Floral Transition in Tobacco (Nicotiana tabacum)

Hai-Yun Wang; Yi Yu; Yong-Duo Sun; Li-Bo Han; Xiao-Min Wu; Jia-He Wu; Gui-Xian Xia; Guo-Qin Liu

doi:10.1016/j.jgg.2015.03.010

Volume 42 Issue 6

Jun. 2015

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Article Navigation > Journal of Genetics and Genomics > 2015 > 42(6): 311-317

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The RING Finger Protein NtRCP1 Is Involved in the Floral Transition in Tobacco (Nicotiana tabacum)

doi: 10.1016/j.jgg.2015.03.010

a
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China
b
State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China

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Corresponding author: E-mail address: xiagx@im.ac.cn (Gui-Xian Xia); E-mail address: liu@cau.edu.cn (Guo-Qin Liu)
Received Date: 2015-01-15
Accepted Date: 2015-03-23
Rev Recd Date: 2015-03-23

Available Online: 2015-04-07

Publish Date: 2015-06-20

Abstract

Abstract

The transition from the vegetative phase to the reproductive phase is a major developmental process in flowering plants. The underlying mechanism controlling this cellular process remains a research focus in the field of plant molecular biology. In the present work, we identified a gene encoding the C3H2C3-type RING finger protein NtRCP1 from tobacco BY-2 cells. Enzymatic analysis demonstrated that NtRCP1 is a functional E3 ubiquitin ligase. In tobacco plants, expression level of NtRCP1 was higher in the reproductive shoot apices than in the vegetative ones. NtRCP1-overexpressing plants underwent a more rapid transition from the vegetative to the reproductive phase and flowered markedly earlier than the wild-type control. Histological analysis revealed that the shoot apical meristem of NtRCP1-overexpressing plants initiated inflorescence primordia precociously compared to the wild-type plant due to accelerated cell division. Overexpression of NtRCP1 in BY-2 suspension cells promoted cell division, which was a consequence of the shortened G2 phase in the cell cycle. Together, our data suggest that NtRCP1 may act as a regulator of the phase transition, possibly through its role in cell cycle regulation, during vegetative/reproductive development in tobacco plant.
- RING finger protein,
- Floral transition,
- Cell division cycle,
- Tobacco

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