STRIPE2 Encodes a Putative dCMP Deaminase that Plays an Important Role in Chloroplast Development in Rice

Jing Xu; Yiwen Deng; Qun Li; Xudong Zhu; Zuhua He

doi:10.1016/j.jgg.2014.05.008

Volume 41 Issue 10

Oct. 2014

Turn off MathJax

Article Contents

Article Navigation > Journal of Genetics and Genomics > 2014 > 41(10): 539-548

PDF( 2851 KB)

STRIPE2 Encodes a Putative dCMP Deaminase that Plays an Important Role in Chloroplast Development in Rice

doi: 10.1016/j.jgg.2014.05.008

a
National Key Laboratory of Plant Molecular Genetics and National Center of Plant Gene Research, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China
b
China National Rice Research Institute, Hangzhou 31006, China

More Information

Corresponding author: E-mail address: ricezxd@126.com (Xudong Zhu); E-mail address: zhhe@sibs.ac.cn (Zuhua He)
Received Date: 2014-03-31
Accepted Date: 2014-05-09
Rev Recd Date: 2014-05-08

Available Online: 2014-06-19

Publish Date: 2014-10-20

Abstract

Abstract

Mutants with abnormal leaf coloration are good genetic materials for understanding the mechanism of chloroplast development and chlorophyll biosynthesis. In this study, a rice mutant st2 (stripe2) with stripe leaves was identified from the γ-ray irradiated mutant pool. The st2 mutant exhibited decreased accumulation of chlorophyll and aberrant chloroplasts. Genetic analysis indicated that the st2 mutant was controlled by a single recessive locus. The ST2 gene was finely confined to a 27-kb region on chromosome 1 by the map-based cloning strategy and a 5-bp deletion inOs01g0765000 was identified by sequence analysis. The deletion happened in the joint of exon 3 and intron 3 and led to new spliced products of mRNA. Genetic complementation confirmed that Os01g0765000 is the ST2 gene. We found that the ST2 gene was expressed ubiquitously. Subcellular localization assay showed that the ST2 protein was located in mitochondria. ST2 belongs to the cytidine deaminase-like family and possibly functions as the dCMP deaminase, which catalyzes the formation of dUMP from dCMP by deamination. Additionally, exogenous application of dUMP could partially rescue the st2 phenotype. Therefore, our study identified a putative dCMP deaminase as a novel regulator in chloroplast development for the first time.
- Chloroplast development,
- dCMP deaminase

FullText(HTML)

References(38)

References

[1]	Arnon, D.I. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in beta vulgaris Plant Physiol., 24 (1949),pp. 1-15
[2]	Bart, R., Chern, M., Park, C.J. et al. A novel system for gene silencing using siRNAs in rice leaf and stem-derived protoplasts Plant Methods, 2 (2006),p. 13
[3]	Boussardon, C., Salone, V., Avon, A. et al. Plant Cell, 24 (2012),pp. 3684-3694
[4]	Carrie, C., Murcha, M.W., Millar, A.H. et al. Plant Mol. Biol., 63 (2007),pp. 97-108
[5]	Chen, H., Cheng, Z., Ma, X. et al. Plant Cell Rep., 32 (2013),pp. 1855-1867
[6]	Chen, M., Galvao, R.M., Li, M. et al. Cell, 141 (2010),pp. 1230-1240
[7]	Collings, D.A., Carter, C.N., Rink, J.C. et al. Plant nuclei can contain extensive grooves and invaginations Plant Cell, 12 (2000),pp. 2425-2440
[8]	Delannoy, E., Le Ret, M., Faivre-Nitschke, E. et al. Plant Cell, 21 (2009),pp. 2058-2071
[9]	Faivre-Nitschke, S.E., Grienenberger, J.M., Gualberto, J.M. Eur. J. Biochem., 263 (1999),pp. 896-903
[10]	Hiei, Y., Ohta, S., Komari, T. et al. Plant J., 6 (1994),pp. 271-282
[11]	Hoch, B., Maier, R.M., Appel, K. et al. Editing of a chloroplast mRNA by creation of an initiation codon Nature, 353 (1991),pp. 178-180
[12]	Jiao, S., Thornsberry, J.M., Elthon, T.E. et al. Biochemical and molecular characterization of photosystem I deficiency in the NCS6 mitochondrial mutant of maize Plant Mol. Biol., 57 (2005),pp. 303-313
[13]	Jung, K.-H., Hur, J., Ryu, C.-H. et al. Characterization of a rice chlorophyll-deficient mutant using the T-DNA gene-trap system Plant Cell Physiol., 44 (2003),pp. 463-472
[14]	Kafer, C., Thornburg, R. J. Plant Biol., 43 (2000),pp. 162-170
[15]	Karcher, D., Bock, R. Identification of the chloroplast adenosine-to-inosine tRNA editing enzyme RNA, 15 (2009),pp. 1251-1257
[16]	Kim, S.R., Yang, J.I., Moon, S. et al. Plant J., 59 (2009),pp. 738-749
[17]	Kusumi, K., Mizutani, A., Nishimura, M. et al. Plant J., 12 (1997),pp. 1241-1250
[18]	Kusumi, K., Sakata, C., Nakamura, T. et al. A plastid protein NUS1 is essential for build-up of the genetic system for early chloroplast development under cold stress conditions Plant J., 68 (2011),pp. 1039-1050
[19]	Li, W., Zhong, S., Li, G. et al. Rice RING protein OsBBI1 with E3 ligase activity confers broad-spectrum resistance against Magnaporthe oryzae by modifying the cell wall defence Cell Res., 21 (2011),pp. 835-848
[20]	Loschelder, H., Schweer, J., Link, B. et al. Plant Physiol., 142 (2006),pp. 642-650
[21]	Moon, J., Zhu, L., Shen, H. et al. Proc. Natl. Acad. Sci. USA, 105 (2008),pp. 9433-9438
[22]	Mullet, J.E. Chloroplast development and gene expression Annu. Rev. Plant Physiol. Plant Mol. Biol., 39 (1988),pp. 475-502
[23]	Pogson, B.J., Albrecht, V. Genetic dissection of chloroplast biogenesis and development: an overview Plant Physiol., 155 (2011),pp. 1545-1551
[24]	Prohaska, K.M., Bennett, R.P., Salter, J.D. et al. The multifaceted roles of RNA binding in APOBEC cytidine deaminase functions Wiley Interdiscip. Rev. RNA (2014)
[25]	Pyo, Y.J., Kwon, K.C., Kim, A. et al. Plant Physiol., 163 (2013),pp. 1844-1858
[26]	Sakamoto, W., Miyagishima, S.Y., Jarvis, P. Chloroplast biogenesis: control of plastid development, protein import, division and inheritance Arabidopsis Book, 6 (2008),p. e0110
[27]	Sakuraba, Y., Rahman, M.L., Cho, S.H. et al. Plant J., 74 (2013),pp. 122-133
[28]	Sargent, R.G., Mathews, C.K. Imbalanced deoxyribonucleoside triphosphate pools and spontaneous mutation rates determined during dCMP deaminase-defective bacteriophage T4 infections J. Biol. Chem., 262 (1987),pp. 5546-5553
[29]	Stephenson, P.G., Fankhauser, C., Terry, M.J. PIF3 is a repressor of chloroplast development Proc. Natl. Acad. Sci. USA, 106 (2009),pp. 7654-7659
[30]	Su, N., Hu, M.L., Wu, D.X. et al. Disruption of a rice pentatricopeptide repeat protein causes a seedling-specific albino phenotype and its utilization to enhance seed purity in hybrid rice production Plant Physiol., 159 (2012),pp. 227-238
[31]	Sugimoto, H., Kusumi, K., Noguchi, K. et al. Plant J., 52 (2007),pp. 512-527
[32]	Sugimoto, H., Kusumi, K., Tozawa, Y. et al. Plant Cell Physiol., 45 (2004),pp. 985-996
[33]	Swiatecka-Hagenbruch, M., Emanuel, C., Hedtke, B. et al. Impaired function of the phage-type RNA polymerase RpoTp in transcription of chloroplast genes is compensated by a second phage-type RNA polymerase Nucleic Acids Res., 36 (2008),pp. 785-792
[34]	Takenaka, M., Zehrmann, A., Verbitskiy, D. et al. RNA editing in plants and its evolution Annu. Rev. Genet., 47 (2013),pp. 335-352
[35]	Vincenzetti, S., Cambi, A., Neuhard, J. et al. Protein Expr. Purif., 15 (1999),pp. 8-15
[36]	Wu, Z., Zhang, X., He, B. et al. A chlorophyll-deficient rice mutant with impaired chlorophyllide esterification in chlorophyll biosynthesis Plant Physiol., 145 (2007),pp. 29-40
[37]	Yoo, S.C., Cho, S.H., Sugimoto, H. et al. Plant Physiol., 150 (2009),pp. 388-401
[38]	Zhang, H., Li, J., Yoo, J.H. et al. Plant Mol. Biol., 62 (2006),pp. 325-337