Drosophila heparan sulfate 3-O sulfotransferase B Null Mutant Is Viable and Exhibits No Defects in Notch Signaling

Yueqin Guo; Ying Feng; Zhouhua Li; Xinhua Lin

doi:10.1016/j.jgg.2014.04.006

Volume 41 Issue 7

Jul. 2014

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Drosophila heparan sulfate 3-O sulfotransferase B Null Mutant Is Viable and Exhibits No Defects in Notch Signaling

doi: 10.1016/j.jgg.2014.04.006

Yueqin Guo^{a, b},
Ying Feng^{c, d},
Zhouhua Li^{a, e},
Xinhua Lin^{a, f}

a
State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
b
University of Chinese Academy of Sciences, Beijing 100049, China
c
School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical University, Wenzhou 325000, China
d
State Key Laboratory of Stress Cell Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China
e
School of Life Sciences, Capital Normal University, Beijing 100048, China
f
Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA

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Corresponding author: E-mail address: zhli@cnu.edu.cn (Zhouhua Li); E-mail address: xinhua.lin@ioz.ac.cn (Xinhua Lin)
Received Date: 2014-01-21
Accepted Date: 2014-04-24
Rev Recd Date: 2014-04-18

Available Online: 2014-05-15

Publish Date: 2014-07-20

Abstract

Abstract

Heparan sulfate proteoglycans (HSPGs) are critically involved in a variety of biological events. The functions of HSPGs are determined by the nature of the core proteins and modifications of heparan sulfate (HS) glycosaminoglycan (GAG) chains. The distinct O-sulfotransferases are important for nonrandom modifications at specific positions. Two HS 3-O sulfotransferase (Hs3st) genes, Hs3st-A and Hs3st-B, were identified in Drosophila. Previous experiments using RNA interference (RNAi) suggested that Hs3st-B was required for Notch signaling. Here, we generated a null mutant of Hs3st-B via ends-out gene targeting and examined its role(s) in development. We found that homozygous Hs3st-B mutants have no neurogenic defects or alterations in the expression of Notch signaling target gene. Thus, our results strongly argue against an essential role for Hs3st-B in Notch signaling. Moreover, we have generated two independent Hs3st-A RNAi lines which worked to deplete Hs3st-A. Importantly, Hs3st-A RNAi combined with Hs3st-B mutant flies did not alter the expression of Notch signaling components, arguing that both Hs3st-A and Hs3st-B were not essential for Notch signaling. The establishment of Hs3st-B mutant and effective Hs3st-A RNAi lines provides essential tools for further studies of the physiological roles of Hs3st-A and Hs3st-B in development and homeostasis.
- heparan sulfate proteoglycans,
- Notch

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References(45)

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