Efficient Gene Targeting in Zebrafish Mediated by a Zebrafish-Codon-Optimized Cas9 and Evaluation of Off-Targeting Effect

Da Liu; Zhanxiang Wang; An Xiao; Yutian Zhang; Wenyuan Li; Yao Zu; Shaohua Yao; Shuo Lin; Bo Zhang

doi:10.1016/j.jgg.2013.11.004

Volume 41 Issue 1

Jan. 2014

Turn off MathJax

Article Contents

Article Navigation > Journal of Genetics and Genomics > 2014 > 41(1): 43-46

PDF( 409 KB)

Efficient Gene Targeting in Zebrafish Mediated by a Zebrafish-Codon-Optimized Cas9 and Evaluation of Off-Targeting Effect

doi: 10.1016/j.jgg.2013.11.004

Da Liu^{a, b, #},
Zhanxiang Wang^{a, #},
An Xiao^{a, #},
Yutian Zhang^a,
Wenyuan Li^a,
Yao Zu^{a, c},
Shaohua Yao^c,
Shuo Lin^{b, c},
Bo Zhang^a

a
Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, College of Life Sciences, Peking University, Beijing 100871, China
b
Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, China
c
Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, CA 90095, USA

More Information

Corresponding author: E-mail address: bzhang@pku.edu.cn (Bo Zhang)
Received Date: 2013-09-11
Accepted Date: 2013-11-14
Rev Recd Date: 2013-11-13

Available Online: 2013-11-22

Publish Date: 2014-01-20

Abstract

These authors contributed equally to this work.

FullText(HTML)

References(14)

References

[1]	Chakraborty, A., Uechi, T., Higa, S. et al. Loss of ribosomal protein L11 affects zebrafish embryonic development through a p53-dependent apoptotic response PLoS ONE, 4 (2009),p. e4152
[2]	Chang, N., Sun, C., Gao, L. et al. Genome editing with RNA-guided Cas9 nuclease in zebrafish embryos Cell Res., 23 (2013),pp. 465-472
[3]	Cong, L., Ran, F.A., Cox, D. et al. Multiplex genome engineering using CRISPR/Cas systems Science, 339 (2013),pp. 819-823
[4]	Gratz, S.J., Cummings, A.M., Nguyen, J.N. et al. Genetics, 194 (2013),pp. 1029-1035
[5]	Huang, P., Xiao, A., Zhou, M. et al. Heritable gene targeting in zebrafish using customized TALENs Nat. Biotechnol, 29 (2011),pp. 699-700
[6]	Huang, P., Zhu, Z., Lin, S. et al. Reverse genetic approaches in zebrafish J. Genet. Genomics, 39 (2012),pp. 421-433
[7]	Hwang, W.Y., Fu, Y., Reyon, D. et al. Efficient genome editing in zebrafish using a CRISPR-Cas system Nat. Biotechnol, 31 (2013),pp. 227-229
[8]	Jao, L.E., Wente, S.R., Chen, W. Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system Proc. Natl. Acad. Sci. USA, 110 (2013),pp. 13904-13909
[9]	Jiang, W., Bikard, D., Cox, D. et al. RNA-guided editing of bacterial genomes using CRISPR-Cas systems Nat. Biotechnol, 31 (2013),pp. 233-239
[10]	Shen, B., Zhang, J., Wu, H. et al. Cell Res., 23 (2013),pp. 720-723
[11]	Wei, C., Liu, J., Yu, Z. et al. TALEN or Cas9–rapid, efficient and specific choices for genome modifications J. Genet. Genomics, 40 (2013),pp. 281-289
[12]	Xiao, A., Wang, Z., Hu, Y. et al. Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish Nucleic Acids Res, 41 (2013),p. e141
[13]	Xiao, A., Wu, Y., Yang, Z. et al. EENdb: a database and knowledge base of ZFNs and TALENs for endonuclease engineering Nucleic Acids Res., 41 (2013),pp. D415-D422
[14]	Zu, Y., Tong, X., Wang, Z. et al. TALEN-mediated precise genome modification by homologous recombination in zebrafish Nat. Methods, 10 (2013),pp. 329-331