Sequence Diversity and Enzyme Activity of Ferric-Chelate Reductase LeFRO1 in Tomato

Danyu Kong; Chunlin Chen; Huilan Wu; Ye Li; Junming Li; Hong-Qing Ling

doi:10.1016/j.jgg.2013.08.002

Volume 40 Issue 11

Nov. 2013

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Sequence Diversity and Enzyme Activity of Ferric-Chelate Reductase LeFRO1 in Tomato

doi: 10.1016/j.jgg.2013.08.002

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, No. 1, West Biechen Road, Chaoyang District, Beijing 100101, China

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Corresponding author: E-mail address: hqling@genetics.ac.cn (Hong-Qing Ling)
Received Date: 2013-07-10
Accepted Date: 2013-08-09
Rev Recd Date: 2013-08-04

Available Online: 2013-09-04

Publish Date: 2013-11-20

Abstract

Abstract

Ferric-chelate reductase which functions in the reduction of ferric to ferrous iron on root surface is a critical protein for iron homeostasis in strategy I plants. LeFRO1 is a major ferric-chelate reductase involved in iron uptake in tomato. To identify the natural variations of LeFRO1 and to assess their effect on the ferric-chelate reductase activity, we cloned the coding sequences of LeFRO1 from 16 tomato varieties collected from different regions, and detected three types of LeFRO1 (LeFRO1^MM, LeFRO1^Ailsa and LeFRO1^Monita) with five amino acid variations at the positions 21, 24, 112, 195 and 582. Enzyme activity assay revealed that the three types of LeFRO1 possessed different ferric-chelate reductase activity (LeFRO1^Ailsa > LeFRO1^MM > LeFRO1^Monita). The 112th amino acid residue Ala of LeFRO1 is critical for maintaining the high activity of ferric-chelate reductase, because modification of this amino acid resulted in a significant reduction of enzyme activity. Further, we showed that the combination of the amino acid residue Ile at the site 24 with Lys at the site 582 played a positive role in the enzyme activity of LeFRO1. In conclusion, the findings are helpful to understand the natural adaptation mechanisms of plants to iron-limiting stress, and may provide new knowledge to select and manipulate LeFRO1 for improving the iron deficiency tolerance in tomato.
- Tomato,
- Iron homeostasis,
- Natural variation,
- Ferric-chelate reductase activity

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References

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