A High-Throughput Method for Screening Arabidopsis Mutants with Disordered Abiotic Stress-Induced Calcium Signal

Zhen Pan; Yang Zhao; Yuan Zheng; Juntao Liu; Xiangning Jiang; Yan Guo

doi:10.1016/j.jgg.2012.04.002

Volume 39 Issue 5

May 2012

Turn off MathJax

Article Contents

Article Navigation > Journal of Genetics and Genomics > 2012 > 39(5): 225-235

PDF( 2907 KB)

A High-Throughput Method for Screening Arabidopsis Mutants with Disordered Abiotic Stress-Induced Calcium Signal

doi: 10.1016/j.jgg.2012.04.002

a
College of Life Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
b
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China
c
National Engineering Laboratory of Tree Breeding, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration, Beijing 100083, China

More Information

Corresponding author: E-mail address: guoyan@cau.edu.cn (Yan Guo)
Received Date: 2012-02-03
Accepted Date: 2012-04-01
Rev Recd Date: 2012-04-01

Available Online: 2012-04-17

Publish Date: 2012-05-20

Abstract

Abstract

It is established that different stresses cause signal-specific changes in cellular Ca²⁺ level, which function as messengers in modulating diverse physiological processes. These calcium signals are important for stress adaptation. Though numbers of downstream components of calcium signal cascades have been identified, upstream events in calcium signal remain elusive, specifically components required for calcium signal generation due to the lack of high-throughput genetic assay. Here, we report the development of an easy and efficient method in a forward genetic screen for Ca²⁺ signals-deficient mutants in Arabidopsis thaliana. Using this method, 121 mutants with disordered NaCl- and H₂O₂-induced Ca²⁺ signals are isolated.
- Mutant screen method,
- Calcium signal,
- Abiotic stress

FullText(HTML)

References(40)

References

[1]	Ali, R., Ma, W., Lemtiri-Chlieh, F. et al. Plant Cell, 19 (2007),pp. 1081-1095
[2]	Allen, G.J., Kuchitsu, K., Chu, S.P. et al. Plant Cell, 11 (1999),pp. 1785-1798
[3]	Arimura, S.-I., Yamamoto, J., Aida, G.P. et al. Frequent fusion and fission of plant mitochondria with unequal nucleoid distribution Proc. Natl. Acad. Sci. USA, 101 (2004),pp. 7805-7808
[4]	Berridge, M.J., Bootman, M.D., Roderick, H.L. Calcium signalling: dynamics, homeostasis and remodelling Nat. Rev. Mol. Cell Biol., 4 (2003),pp. 517-529
[5]	Davenport, R. Glutamate receptors in plants Ann. Bot., 90 (2002),pp. 549-557
[6]	Frohnmeyer, H., Loyall, L., Blatt, M.R. et al. Plant J., 20 (1999),pp. 109-117
[7]	Furuichi, T., Cunningham, K.W., Muto, S. Plant Cell Physiol., 42 (2001),pp. 900-905
[8]	Gilroy, S., Read, N.D., Trewavas, A.J. Elevation of cytoplasmic calcium by caged calcium or caged inositol trisphosphate initiates stomatal closure Nature, 346 (1990),pp. 769-771
[9]	Gong, D., Guo, Y., Schumaker, K.S. et al. Plant Physiol., 134 (2004),pp. 919-926
[10]	Haeseleer, F., Imanishi, Y., Sokal, I. et al. Calcium-binding proteins: intracellular sensors from the calmodulin superfamily Biochem. Biophys. Res. Commun., 290 (2002),pp. 615-623
[11]	Han, S., Tang, R., Anderson, L.K. et al. Nature, 425 (2003),pp. 196-200
[12]	Hepler, P.K. Calcium: a central regulator of plant growth and development Plant Cell, 17 (2005),pp. 2142-2155
[13]	Hetherington, A.M., Brownlee, C. Annu. Rev. Plant. Biol., 55 (2004),pp. 401-427
[14]	Hrabak, E.M., Chan, C.W., Gribskov, M. et al. Plant Physiol., 132 (2003),pp. 666-680
[15]	Jammes, F., Hu, H.-C., Villiers, F. et al. Calcium-permeable channels in plant cells FEBS J., 278 (2011),pp. 4262-4276
[16]	Köhler, C., Merkle, T., Neuhaus, G. Plant J., 18 (1999),pp. 97-104
[17]	Knight, H., Trewavas, A.J., Knight, M.R. Plant Cell, 8 (1996),pp. 489-503
[18]	Knight, H., Trewavas, A.J., Knight, M.R. Plant J., 12 (1997),pp. 1067-1078
[19]	Knight, M.R., Campbell, A.K., Smith, S.M. et al. Transgenic plant aequorin reports the effects of touch and cold-shock and elicitors on cytoplasmic calcium Nature, 352 (1991),pp. 524-526
[20]	Krysan, P.J., Young, J.C., Sussman, M.R. Plant Cell, 11 (1999),pp. 2283-2290
[21]	Kudla, J., Batistič, O., Hashimoto, K. Calcium signals: the lead currency of plant information processing Plant Cell, 22 (2010),pp. 541-563
[22]	Leckie, C.P., McAinsh, M.R., Allen, G.J. et al. Abscisic acid-induced stomatal closure mediated by cyclic ADP-ribose Proc. Natl. Acad. Sci. USA, 95 (1998),pp. 15837-15842
[23]	Lemtiri-Chlieh, F., MacRobbie, E.A.C., Webb, A.A.R. et al. Inositol hexakisphosphate mobilizes an endomembrane store of calcium in guard cells Proc. Natl. Acad. Sci. USA, 100 (2003),pp. 10091-10095
[24]	Liu, Y.-G., Mitsukawa, N., Oosumi, T. et al. Plant J., 8 (1995),pp. 457-463
[25]	Malli, R., Frieden, M., Osibow, K. et al. J. Biol. Chem., 278 (2003),pp. 44769-44779
[26]	Michard, E., Lima, P.T., Borges, F. et al. Science, 332 (2011),pp. 434-437
[27]	Mori, I.C., Schroeder, J.I. Plant Physiol., 135 (2004),pp. 702-708
[28]	Navazio, L., Bewell, M.A., Siddiqua, A. et al. Calcium release from the endoplasmic reticulum of higher plants elicited by the NADP metabolite nicotinic acid adenine dinucleotide phosphate Proc. Natl. Acad. Sci. USA, 97 (2000),pp. 8693-8698
[29]	Pei, Z.-M., Murata, Y., Benning, G. et al. Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells Nature, 406 (2000),pp. 731-734
[30]	Qi, Z., Stephens, N.R., Spalding, E.P. Plant Physiol., 142 (2006),pp. 963-971
[31]	Quan, R., Lin, H., Mendoza, I. et al. Plant Cell, 19 (2007),pp. 1415-1431
[32]	Rentel, M.C., Knight, M.R. Plant Physiol., 135 (2004),pp. 1471-1479
[33]	Stam, M., Mol, J.N.M., Kooter, J.M. Review article: the silence of genes in transgenic plants Ann. Bot., 79 (1997),pp. 3-12
[34]	Szabados, L., Kovacs, I., Oberschall, A. et al. Plant J., 32 (2002),pp. 233-242
[35]	Verslues, P.E., Guo, Y., Dong, C.-H. et al. Plant J., 47 (2006),pp. 776-787
[36]	Weigel, D., Ahn, J.H., Blázquez, M.A. et al. Plant Physiol., 122 (2000),pp. 1003-1014
[37]	Weinl, S., Held, K., Schlücking, K. et al. New Phytol., 179 (2008),pp. 675-686
[38]	Xiong, L., Schumaker, K.S., Zhu, J.-K. Cell signaling during cold, drought, and salt stress Plant Cell, 14 (2002),pp. S165-S183
[39]	Yamada, A., Yamamoto, T., Yoshimura, Y. et al. Biochim. Biophys. Acta., 1787 (2009),pp. 1486-1491
[40]	Zhu, J.-K. Regulation of ion homeostasis under salt stress Curr. Opin. Plant. Biol., 6 (2003),pp. 441-445