Identification of quantitative trait loci associated with salt tolerance at seedling stage from Oryza rufipogon

Lei Tian; Lubin Tan; Fengxia Liu; Hongwei Cai; Chuanqing Sun

doi:10.1016/j.jgg.2011.11.005

Volume 38 Issue 12

Dec. 2011

Turn off MathJax

Article Contents

Article Navigation > Journal of Genetics and Genomics > 2011 > 38(12): 593-601

PDF( 1043 KB)

Identification of quantitative trait loci associated with salt tolerance at seedling stage from Oryza rufipogon

doi: 10.1016/j.jgg.2011.11.005

State Key Laboratory of Plant Physiology and Biochemistry, National Centre for Evaluation of Agricultural Wild Plant (Rice), Laboratory of Crop Heterosis and Utilization of Ministry of Education; Beijing Key Laboratory of Crop Genetic Improvement and Genome of Ministry of Agriculture, Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100193, China

More Information

Corresponding author: E-mail address: suncq@cau.edu.cn (Chuanqing Sun)
Received Date: 2011-10-12
Accepted Date: 2011-11-25
Rev Recd Date: 2011-11-24

Available Online: 2011-11-30

Publish Date: 2011-12-20

Abstract

Abstract

Soil salinity is one of the major abiotic stresses affecting plant growth and crop production. In the present study, salt tolerance at rice seedling stage was evaluated using 87 introgression lines (ILs), which were derived from a cross between an elite indica cultivar Teqing and an accession of common wild rice (Oryza rufipogon Griff.). Substantial variation was observed for four traits including salt tolerance score (STS), relative root dry weight (RRW), relative shoot dry weight (RSW) and relative total dry weight (RTW). STS was significantly positively correlated with all other three traits. A total of 15 putative quantitative trait loci (QTLs) associated with these four traits were detected using single-point analysis, which were located on chromosomes 1, 2, 3, 6, 7, 9 and 10 with 8%–26% explaining the phenotypic variance. TheO. rufipogon-derived alleles at 13 QTLs (86.7%) could improve the salt tolerance in the Teqing background. Four QTL clusters affecting RRW, RSW and RTW were found on chromosomes 6, 7, 9 and 10, respectively. Among these four QTL clusters, a major cluster including three QTLs (qRRW10, qRSW10 and qRTW10) was found near the maker RM271 on the long arm of chromosome 10, and the O. rufipogon-derived alleles at these three loci increased RRW, RSW and RTW with additive effects of 22.7%, 17.3% and 18.5%, respectively, while the phenotypic variance explained by these three individual QTLs for the three traits varied from 19% to 26%. In addition, several salt tolerant ILs were selected and could be used for identifying and utilizing favorable salt tolerant genes from common wild rice and used in the salt tolerant rice breeding program.
- Introgression lines,
- Salt tolerance,
- Seedling stage,
- Quantitative trait locus

FullText(HTML)

References(37)

References

[1]	Ammar, M.H.M., Pandit, A., Singh, R.K. et al. J. Plant Biochem. Biotechnol., 18 (2009),pp. 139-150
[2]	Bal, A.R., Dutt, S.K. Plant Soil, 92 (1986),pp. 399-404
[3]	Brar, D.S., Khush, G.S. Alien introgression in rice Plant Mol. Biol., 35 (1997),pp. 35-47
[4]	Cheng, Y.W., Qi, Y.C., Zhu, Q. et al. New changes in the plasma-membrane-associated proteome of rice roots under salt stress Proteomics, 9 (2009),pp. 3100-3114
[5]	Cho, S.K., Jeung, J.U., Kang, K.H. et al. Mol. Cells, 17 (2004),pp. 230-236
[6]	Farooq, S., Asghar, M., Iqbal, N. et al. Int. Rice Res. Newsl., 17 (1992),p. 16
[7]	Flowers, T.J., Koyama, M.L., Flowers, S.A. et al. QTL: their place in engineering tolerance of rice to salinity J. Exp. Bot., 51 (2000),pp. 99-106
[8]	Flowers, T.J., Yeo, A.R. New Phytol., 81 (1981),pp. 363-373
[9]	Fu, Q., Zhang, P.J., Tan, L.B. et al. J. Genet. Genomics, 37 (2010),pp. 147-157
[10]	Gregorio, G.B., Senadhira, D., Mendoza, R.D. et al. Progress in breeding for salinity tolerance and associated abiotic stresses in rice Field Crop Res., 76 (2002),pp. 91-101
[11]	Haq, T.U., Gorham, J., Akhtar, J. et al. Dynamic quantitative trait loci for salt stress components on chromosome 1 of rice Funct. Plant Biol., 37 (2010),pp. 634-645
[12]	He, G.M., Luo, X.J., Tian, F. et al. Haplotype variation in structure and expression of a gene cluster associated with a quantitative trait locus for improved yield in rice Genome Res., 16 (2006),pp. 618-626
[13]	Hossain, M.A., Cho, J., Han, M. et al. The ABRE-binding bZIP transcription factor OsABF2 is a positive regulator of abiotic stress and ABA signaling in rice J. Plant Physiol., 167 (2010),pp. 1512-1520
[14]	Huang, X.Y., Chao, D.Y., Gao, J.P. et al. Gene Dev., 23 (2009),pp. 1805-1817
[15]	Koyama, M.L., Levesley, A., Koebner, R.M.D. et al. Quantitative trait loci for component physiological traits determining salt tolerance in rice Plant Physiol., 125 (2001),pp. 406-422
[16]	Lee, S.Y., Ahn, J.H., Cha, Y.S. et al. Mapping QTLs related to salinity tolerance of rice at the young seedling stage Plant Breed., 126 (2007),pp. 43-46
[17]	Li, D.J., Sun, C.Q., Fu, Y.C. et al. Chin. Sci. Bull., 18 (2002),pp. 1533-1537
[18]	Lin, H.X., Zhu, M.Z., Yano, M. et al. Theor. Appl. Genet., 108 (2004),pp. 253-260
[19]	Manly, K.F., Cudmore, J.R.H., Meer, J.M. Map Manager QTX, cross-platform software for genetic mapping Mamm. Genome, 12 (2001),pp. 930-932
[20]	Moncada, P., Martinez, C.P., Borrero, J. et al. Theor. Appl. Genet., 102 (2001),pp. 41-52
[21]	Nguyen, B.D., Brar, D.S., Bui, B.C. et al. Theor. Appl. Genet., 106 (2003),pp. 583-593
[22]	Pandit, A., Rai, V., Bal, S. et al. Mol. Genet. Genomics, 284 (2010),pp. 121-136
[23]	Prasad, S.R., Bagali, P.G., Hittalmani, S. et al. Molecular mapping of quantitative trait loci associated with seedling tolerance of salt stress in rice Curr. Sci., 78 (1999),pp. 162-164
[24]	Ram, T., Majumder, N.D., Mishra, B. et al. Curr. Sci., 92 (2007),pp. 225-230
[25]	Ren, Z.H., Gao, J.P., Li, L.G. et al. A rice quantitative trait locus for salt tolerance encodes a sodium transporter Nat. Genet., 37 (2005),pp. 1141-1146
[26]	Sabouri, H., Rezai, A.M., Moumeni, A. et al. QTLs mapping of physiological traits related to salt tolerance in young rice seedlings Biol. Plantarum, 53 (2009),pp. 657-662
[27]	Second, G. Jpn. J. Genet., 57 (1982),pp. 25-57
[28]	Septiningsih, E.M., Prasetiyono, J., Lubis, E. et al. Theor. Appl. Genet., 107 (2003),pp. 1419-1432
[29]	Sun, C.Q., Wang, X.K., Yoshimura, A. et al. Theor. Appl. Genet., 102 (2001),pp. 157-162
[30]	Takehisa, H., Shimodate, T., Fukuta, Y. et al. Identification of quantitative trait loci for plant growth of rice in paddy field flooded with salt water Field Crop Res., 89 (2004),pp. 85-95
[31]	Tan, L.B., Liu, F.X., Xue, W. et al. J. Integr. Plant Biol., 49 (2007),pp. 871-884
[32]	Thomson, M.J., Tai, T.H., McClung, A.M. et al. Theor. Appl. Genet., 107 (2003),pp. 479-493
[33]	Tian, F., Li, D.J., Fu, Q. et al. Theor. Appl. Genet., 112 (2006),pp. 570-580
[34]	Wang, Z.Y., Second, G., Tanksley, S.D. Theor. Appl. Genet., 83 (1992),pp. 565-581
[35]	Yeo, A.R., Yeo, M.E., Flower, S.A. et al. Theor. Appl. Genet., 79 (1990),pp. 377-384
[36]	Yoshida, S., Forno, D.A., Cock, J.H. et al.
[37]	Zang, J.P., Sun, Y., Wang, Y. et al. Dissection of genetic overlap of salt tolerance QTLs at the seedling and tillering stages using backcross introgression lines in rice Sci. China Ser. C Life Sci., 51 (2008),pp. 583-591