Gene expression profiling of Puccinia striiformis f. sp. tritici during development reveals a highly dynamic transcriptome

Xueling Huang; Xianming Chen; Tristan Coram; Meinan Wang; Zhensheng Kang

doi:10.1016/j.jgg.2011.07.004

Volume 38 Issue 8

Aug. 2011

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Gene expression profiling of Puccinia striiformis f. sp. tritici during development reveals a highly dynamic transcriptome

doi: 10.1016/j.jgg.2011.07.004

a
State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, PR China
b
Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USA
c
United States Department of Agriculture, Agricultural Research Service, Wheat Genetics, Quality, Physiology and Disease Research Unit, Pullman, WA 99164-6430, USA

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Corresponding author: E-mail address: xianming@wsu.edu (Xianming Chen)
Received Date: 2011-05-02
Accepted Date: 2011-07-15
Rev Recd Date: 2011-07-12

Available Online: 2011-07-23

Publish Date: 2011-08-20

Abstract

Abstract

Puccinia striiformis f. sp. tritici (Pst) causes stripe rust, one of the most important diseases of wheat worldwide. cDNA libraries had been constructed from urediniospores, germinated urediniospores and haustoria. However, little is known about the expression patterns of the genes related to the infection process and sporulation of the pathogen. In this study, a custom oligonucleotide microarray was constructed using sequences of 442 gene transcripts selected fromPst cDNA libraries. The expression patterns of the genes were determined by hybridizing the microarray with cDNA from Pst in vitro and Pst-infected wheat leaves. The time course study identified 55 transcripts that were differentially expressed during the infection process in a compatible interaction. They were identified to have functions related to the following biological processes, including carbohydrate and lipid metabolism, energy, cell signaling, protein synthesis, cell structure and division. In an incompatible interaction, 17 transcripts of the pathogen were differentially expressed in resistant wheat leaves inoculated with an avirulent Pst race, ten of which had similar expression patterns to those in the compatible interaction. Several candidates for pathogenicity and virulence/avirulence related genes were also identified. The results of quantitative real-time PCR validated the expression patterns of some selected genes. The study demonstrates that the custom oligonucleotide microarray technology is useful to determine the expression patterns of the pathogen genes involved in different types of the host–pathogen interactions and stages of development.
- Stripe rust,
- Yellow rust,
- Expression profiling,
- Microarray,
- Wheat

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References(47)

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