Establishment of novel embryonic stem (ES) cell lines from OG2/rtTA blastocysts

Hui Yao; Yonghua Jiang; Yu Zhang; Wenqiang Liu; Bo Huang; Xiaodong Wang; Shaorong Gao

doi:10.1016/j.jgg.2011.05.007

Volume 38 Issue 7

Jul. 2011

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Article Navigation > Journal of Genetics and Genomics > 2011 > 38(7): 289-295

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Establishment of novel embryonic stem (ES) cell lines from OG2/rtTA blastocysts

doi: 10.1016/j.jgg.2011.05.007

a
College of Life Science, Beijing Normal University, Beijing 100875, China
b
National Institute of Biological Sciences, NIBS, Beijing 102206, China

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Corresponding author: E-mail address: gaoshaorong@nibs.ac.cn (Shaorong Gao)
Received Date: 2011-04-23
Accepted Date: 2011-05-26
Rev Recd Date: 2011-05-25

Available Online: 2011-06-20

Publish Date: 2011-07-20

Abstract

Abstract

Embryonic stem (ES) cells derived from the pre-implantation blastocyst-stage embryos have been widely used to investigate the molecular events determining pluripotency and cell lineage differentiation. As the first discovered ES-specific transcription factor, Oct4 has been considered as the core pluripotency factor of ES cells. In the present study, we successfully established seven ES lines from the blastocysts collected from female OG2 (Oct4-GFP transgenic) mice, which have been crossed with male rtTA transgenic mice. The pluripotency of the ES cell lines can be visualized by the expression of Oct4-GFP under fluorescent microscopy and germ-line transmission capability has been further confirmed. More importantly, the presence of rtTA could induce transgene’s expression with the help of doxycycline. Therefore, these ES cell lines provide an excellent tool to further discover novel factors affecting pluripotency and to investigate the molecular mechanism of reprogramming in defined transcription factors mediated nuclear reprogramming.
- Embryonic stem (ES) cells,
- Oct4,
- rtTA

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References(27)

References

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