A rice mutant displaying a heterochronically elongated internode carries a 100 kb deletion

Mika Hayashi-Tsugane; Masahiko Maekawa; Qian Qian; Hirokazu Kobayashi; Shigeru Iida; Kazuo Tsugane

doi:10.1016/j.jgg.2011.02.004

Volume 38 Issue 3

Mar. 2011

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A rice mutant displaying a heterochronically elongated internode carries a 100 kb deletion

doi: 10.1016/j.jgg.2011.02.004

a
National Institute for Basic Biology, Okazaki 444-8585, Japan
b
Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka 422-8526, Japan
c
Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan
d
Global Center of Excellence Program, University of Shizuoka, Shizuoka 422-8526, Japan
e
Graduate School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422-8526, Japan
f
The Graduate University for Advanced Studies (Sokendai), Okazaki 444-8585, Japan

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Corresponding author: E-mail address: tsugane@nibb.ac.jp (Kazuo Tsugane)
Received Date: 2010-10-10
Accepted Date: 2011-01-27
Rev Recd Date: 2011-01-18

Available Online: 2011-03-16

Publish Date: 2011-03-20

Abstract

Abstract

We have isolated a recessive rice mutant, designated as indeterminate growth (ing), which displays creeping and apparent heterochronic phenotypes in the vegetative period with lanky and winding culms. Rough mapping and subsequent molecular characterization revealed that the ing mutant carries a large deletion, which corresponds to a 103 kb region in the Nipponbare genome, containing nine annotated genes on chromosome 3. Of these annotated genes, the SLR1 gene encoding a DELLA protein is the only one that is well characterized in its function, and its null mutation, which is caused by a single base deletion in the middle of the intronless SLR1 gene, confers a slender phenotype that bears close resemblance to the ing mutant phenotype. The primary cause of the ing mutant phenotype is the deletion of the SLR1 gene, and the ing mutant appears to be the first characterized mutant having the entire SLR1 sequence deleted. Our results also suggest that the deleted region of 103 kb does not contain an indispensable gene, whose dysfunction must result in a lethal phenotype.
- Heterochronic,
- Elongated internode,
- Large deletion,
- Map-based cloning,
- Rice genome

Permanent address: China National Rice Research Institute, 310006 Hangzhou, China.

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References(22)

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