Derivation and characterization of human embryonic stem cell lines from the Chinese population

Zhao Wu; Hui Li; Lingjun Rao; Lixiazi He; Lei Bao; Jing Liao; Chun Cui; Zhenyu Zuo; Qiao Li; Huimin Dai; Lei Qian; Qing Tian; Lei Xiao; Xiaojun Tan

doi:10.1016/j.jcg.2010.12.006

Volume 38 Issue 1

Jan. 2011

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Article Navigation > Journal of Genetics and Genomics > 2011 > 38(1): 13-20

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Derivation and characterization of human embryonic stem cell lines from the Chinese population

doi: 10.1016/j.jcg.2010.12.006

Zhao Wu^{a, #},
Hui Li^{b, #},
Lingjun Rao^{a, #},
Lixiazi He^a,
Lei Bao^a,
Jing Liao^a,
Chun Cui^c,
Zhenyu Zuo^b,
Qiao Li^b,
Huimin Dai^a,
Lei Qian^a,
Qing Tian^a,
Lei Xiao^c,
Xiaojun Tan^b

a
Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Cell Bank, Stem Cell Bank, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
b
Xiangtan Center Hospital, Hunan 411100, China
c
College of Animal Sciences, Zhejiang University, Hangzhou 310058, China

More Information

Corresponding author: E-mail address: leixiao@zju.edu.cn (Lei Xiao); E-mail address: changda0680@sina.com (Xiaojun Tan)
Received Date: 2010-07-17
Accepted Date: 2010-11-05
Rev Recd Date: 2010-11-01

Available Online: 2011-02-19

Publish Date: 2011-01-20

Abstract

Abstract

Human embryonic stem cells (hESCs) can self-renew indefinitely and differentiate into all cell types in the human body. Therefore, they are valuable in regenerative medicine, human developmental biology and drug discovery. A number of hESC lines have been derived from the Chinese population, but limited of them are available for research purposes. Here we report the derivation and characterization of two hESC lines derived from human blastocysts of Chinese origin. These hESCs express alkaline phosphatase and hESC-specific markers, including Oct4, Nanog, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81. They also have high levels of telomerase activity and normal karyotypes. These cells can form embryoid body in vitro and can be differentiated into all three germ layers in vivo by teratoma formation. The newly established hESCs will be distributed for research purposes. The availability of hESC lines from the Chinese population will facilitate studies on the differences in hESCs from different ethnic groups.
- Human embryonic stem cells,
- Pluripotency,
- Chinese population

These authors contributed equally to this work.

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References(40)

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