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Volume 38 Issue 1
Jan.  2011
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Article Navigation >  Journal of Genetics and Genomics  > 2011  >  38(1): 1-11

TRIM-9 functions in the UNC-6/UNC-40 pathway to regulate ventral guidance

doi: 10.1016/j.jcg.2010.12.004
  • a 

    Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

  • b 

    State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

  • c 

    Graduate School of Chinese Academy of Sciences, Beijing 100049, China

More Information
  • Corresponding author: E-mail address: xhuang@genetics.ac.cn (Xun Huang)
  • Received Date: 2010-11-26
  • Accepted Date: 2010-12-07
  • Rev Recd Date: 2010-12-06
    • Available Online: 2011-02-19
  • Publish Date: 2011-01-20
    Abstract
  • TRIpartite Motif (TRIM) family proteins are ring finger domain-containing, multi-domain proteins implicated in many biological processes. Members of the TRIM-9/C-I subfamily of TRIM proteins, including TRIM-9, MID1 and MID2, have neuronal functions and are associated with neurological diseases. To explore whether the functions of C-I TRIM proteins are conserved in invertebrates, we analyzed Caenorhabditis elegans and Drosophila trim-9 mutants. C. elegans trim-9 mutants exhibit defects in the ventral guidance of hermaphrodite specific neuron (HSN) and the touch neuron AVM. Further genetic analyses indicate that TRIM-9 participates in the UNC-6-UNC-40 attraction pathway. Asymmetric distribution of UNC-40 during HSN development is normal in trim-9 mutants. However, the asymmetric localization of MIG-10, a downstream effector of UNC-40, is abolished in trim-9 mutants. These results suggest that TRIM-9 functions upstream of MIG-10 in the UNC-40 pathway. Moreover, we showed that TRIM-9 exhibits E3 ubiquitin ligase activity in vitro and this activity is important for TRIM-9 function in vivo. Additionally, we found that Drosophila trim-9 is required for the midline attraction of a group of sensory neuron axons. Over-expression of the Netrin/UNC-6 receptor Frazzled suppresses the guidance defects in trim-9 mutants. Our study reveals an evolutionarily conserved function of TRIM-9 in the UNC-40/Frazzled-mediated UNC-6/Netrin attraction pathway.
    • These authors contributed equally to this work.
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    • References(43)
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