Characterization of seedling proteomes and development of markers to distinguish the Brassica A and C genomes

Fang Kong; Cailin Ge; Xiaoping Fang; Rod J. Snowdon; Youping Wang

doi:10.1016/S1673-8527(09)60051-5

Volume 37 Issue 5

May 2010

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Article Navigation > Journal of Genetics and Genomics > 2010 > 37(5): 333-340

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Characterization of seedling proteomes and development of markers to distinguish the Brassica A and C genomes

doi: 10.1016/S1673-8527(09)60051-5

a
College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China
b
Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China
c
Institute for Plant Breeding and Crop Science I, Justus Liebig University, Heinrich-Buff-Ring 26-32, Giessen 35392, Germany

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Corresponding author: E-mail address: wangyp@yzu.edu.cn (Youping Wang)
Received Date: 2010-02-11
Accepted Date: 2010-04-06
Rev Recd Date: 2010-03-30

Available Online: 2010-05-31

Publish Date: 2010-05-20

Abstract

Abstract

The diploid species Brassica rapa (genome AA) and B. oleracea (genome CC) were compared by full-scale proteome analyses of seedling. A total of 28.2% of the proteins was common to both species, indicating the existence of a basal or ubiquitous proteome. However, a number of discriminating proteins (32.0%) and specific proteins (39.8%) of the Brassica A and C genomes, respectively, were identified, which could represent potentially species-specific functions. Based on these A or C genome-specific proteins, a number of PCR-based markers to distinguish B. rapa and B. oleracea species were also developed.
- Brassica rapa,
- Brassica oleracea,
- proteomics,
- molecular marker

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References(27)

References

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