SNL fibroblast feeder layers support derivation and maintenance of human induced pluripotent stem cells

Chuanying Pan; Amy Hicks; Xuan Guan; Hong Chen; Colin E. Bishop

doi:10.1016/S1673-8527(09)60042-4

Volume 37 Issue 4

Apr. 2010

Turn off MathJax

Article Contents

Abstract

References

Article Navigation > Journal of Genetics and Genomics > 2010 > 37(4): 241-248

PDF( 566 KB)

SNL fibroblast feeder layers support derivation and maintenance of human induced pluripotent stem cells

doi: 10.1016/S1673-8527(09)60042-4

a
College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling 712100, China
b
Wake Forest University Institute for Regenerative Medicine, Wake Forest University, Winston-Salem, NC 27101, USA

More Information

Corresponding author: E-mail address: chenhong1212@263.net (Hong Chen); E-mail address: cbishop@wfubmc.edu (Colin E. Bishop)
Received Date: 2009-12-18
Accepted Date: 2010-01-26
Rev Recd Date: 2010-01-22

Available Online: 2010-05-01

Publish Date: 2010-04-20

Abstract

Abstract

Induced pluripotent stem (iPS) cells can be derived from human somatic cells by cellular reprogramming. This technology provides a potential source of non-controversial therapeutic cells for tissue repair, drug discovery, and opportunities for studying the molecular basis of human disease. Normally, mouse embryonic fibroblasts (MEFs) are used as feeder layers in the initial derivation of iPS lines. The purpose of this study was to determine whether SNL fibroblasts can be used to support the growth of human iPS cells reprogrammed from somatic cells using lentiviral expressed reprogramming factors. In our study, iPS cells expressed common pluripotency markers, displayed human embryonic stem cells (hESCs) morphology and unmethylated promoters ofNANOG and OCT4. These data demonstrate that SNL feeder cells can support the derivation and maintenance of human iPS cells.
- induced pluripotent stem cells,
- derivation,
- maintenance,
- SNL,
- feeder cells

FullText(HTML)

References(19)

References

[1]	Cabrera, C.M., Cobo, F., Nieto, A. et al. Strategies for preventing immunologic rejection of transplanted human embryonic stem cells Cytotherapy, 8 (2006),pp. 517-518
[2]	Cheng, L., Hammond, H., Ye, Z. et al. Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture Stem Cells, 21 (2003),pp. 131-142
[3]	Freberg, C.T., Dahl, J.A., Timoskainen, S. et al. Mol. Biol. Cell, 18 (2007),pp. 1543-1553
[4]	Genbacev, O., Krtolica, A., Zdravkovic, T. et al. Serum-free derivation of human embryonic stem cell lines on human placental fibroblast feeders Fertil. Steril., 83 (2005),pp. 1517-1529
[5]	McMahon, A.P., Bradley, A. Cell, 62 (1990),pp. 1073-1085
[6]	Nichols, J., Zevnik, B., Anastassiadis, K. et al. Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4 Cell, 95 (1998),pp. 379-391
[7]	Park, J.H., Kim, S.J., Oh, E.J. et al. Establishment and maintenance of human embryonic stem cells on STO, a permanently growing cell line Biol. Reprod., 69 (2003),pp. 2007-2014
[8]	Reubinoff, B.E., Pera, M.F., Fong, C.Y. et al. Nat. Biotechnol., 18 (2000),pp. 399-404
[9]	Richards, M., Fong, C.Y., Chan, W.K. et al. Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells Nat. Biotechnol., 20 (2002),pp. 933-936
[10]	Richards, M., Tan, S., Fong, C.Y. et al. Comparative evaluation of various human feeders for prolonged undifferentiated growth of human embryonic stem cells Stem Cells, 21 (2003),pp. 546-556
[11]	Saxena, S., Hanwate, M., Deb, K. et al. FGF2 secreting human fibroblast feeder cells: a novel culture system for human embryonic stem cells Mol. Reprod. Dev., 75 (2008),pp. 1523-1532
[12]	Simon, C., Escobedo, C., Valbuena, D. et al. First derivation in Spain of human embryonic stem cell lines: use of long-term cryopreserved embryos and animal-free conditions Fertil. Steril., 83 (2005),pp. 246-249
[13]	Stojkovic, P., Lako, M., Stewart, R. et al. An autogeneic feeder cell system that efficiently supports growth of undifferentiated human embryonic stem cells Stem Cells, 23 (2005),pp. 306-314
[14]	Takahashi, K., Tanabe, K., Ohnuki, M. et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors Cell, 131 (2007),pp. 861-872
[15]	Takahashi, K., Yamanaka, S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors Cell, 126 (2006),pp. 663-676
[16]	Thomson, J.A., Itskovitz-Eldor, J., Shapiro, S.S. et al. Embryonic stem cell lines derived from human blastocysts Science, 282 (1998),pp. 1145-1147
[17]	Unger, C., Skottman, H., Blomberg, P. et al. Good manufacturing practice and clinical-grade human embryonic stem cell lines Hum. Mol. Genet., 17 (2008),pp. R48-R53
[18]	Xu, C., Jiang, J., Sottile, V. et al. Immortalized fibroblast-like cells derived from human embryonic stem cells support undifferentiated cell growth Stem Cells, 22 (2004),pp. 972-980
[19]	Yu, J., Vodyanik, M.A., Smuga-Otto, K. et al. Induced pluripotent stem cell lines derived from human somatic cells Science, 318 (2007),pp. 1917-1920