A novel marker for the platyfish (Xiphophorus maculatus) W chromosome is derived from a Polinton transposon

Qingchun Zhou; Ingo Braasch; Alexander Froschauer; Astrid Böhne; Christina Schultheis; Manfred Schartl; Jean-Nicolas Volff

doi:10.1016/S1673-8527(09)60036-9

Volume 37 Issue 3

Mar. 2010

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Article Navigation > Journal of Genetics and Genomics > 2010 > 37(3): 181-188

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A novel marker for the platyfish (Xiphophorus maculatus) W chromosome is derived from a Polinton transposon

doi: 10.1016/S1673-8527(09)60036-9

a
Biofuture Research Group, Physiologische Chemie I, Biozentrum, University of Würzburg, Würzburg 97074, Germany
b
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Huazhong Normal University, Wuhan 97074, China
c
Institut für Zoologie, Technische Universität Dresden, Dresden 01062, Germany
d
Institut de Génomique Fonctionnelle de Lyon, Université de Lyon, Institut Fédératif Biosciences Gerland Lyon Sud, Université Lyon 1, CNRS, INRA, Ecole Normale Supérieure de Lyon, Lyon 69364, France

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Corresponding author: E-mail address: qczhou@mail.ccnu.edu.cn (Qingchun Zhou); E-mail address: Jean-Nicolas.Volff@ens-lyon.fr (Jean-Nicolas Volff)
Received Date: 2009-06-15
Accepted Date: 2009-11-25
Rev Recd Date: 2009-11-13

Available Online: 2010-03-27

Publish Date: 2010-03-20

Abstract

Abstract

A consensus sequence, encoding a putative DNA polymerase type B derived from a Polinton transposon, was assembled from the sex determination region of Xiphophorus maculatus. This predicted protein, which is 1,158 aa in length, contains a DNA_pol_B_2 domain and a DTDS motif. The DNA polymerase type B gene has about 10 copies in the haploid X. maculatus genome with one Y-specific copy. Interestingly, it has specific copies on the W chromosome in the X. maculatus Usumacinta strain (sex determination with female heterogamety), which represent new markers for this type of sex chromosome in platyfish. This marker with W- and Y-specific copies suggests relationship between different types of gonosomes and allows comparing male and female heterogameties in the platyfish. Further molecular analysis of the DNA polymerase type B gene in X. maculatus will shed new light on the evolution of sex chromosomes in platyfish.
- DNA polymerase type B,
- chromosome marker,
- Xiphophorus maculatus

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References(34)

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