Identification of differential genes in the ovary relative to the testis and their expression patterns in half-smooth tongue sole (Cynoglossus semilaevis)

Yeying Sun; Quanqi Zhang; Jie Qi; Yanjie Chen; Qiwang Zhong; Chunmei Li; Yan Yu; Shuo Li; Zhigang Wang

doi:10.1016/S1673-8527(09)60032-1

Volume 37 Issue 2

Feb. 2010

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Article Navigation > Journal of Genetics and Genomics > 2010 > 37(2): 137-145

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Identification of differential genes in the ovary relative to the testis and their expression patterns in half-smooth tongue sole (Cynoglossus semilaevis)

doi: 10.1016/S1673-8527(09)60032-1

a
Key Laboratory of Marine Genetics and Breeding (College of Marine Life Science, Ocean University of China), Ministry of Education, Qingdao 266003, China
b
College of Pharmacy, Binzhou Medical University, Yantai 264003, China

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Corresponding author: E-mail address: qzhang@ouc.edu.cn (Quanqi Zhang)
Received Date: 2009-07-11
Accepted Date: 2009-11-26
Rev Recd Date: 2009-11-23

Available Online: 2010-03-18

Publish Date: 2010-02-20

Abstract

Abstract

Half-smooth tongue sole (Cynoglossus semilaevis) is a rare marine flatfish whose mature ovary and testis greatly differ in volume and weight. The length and weight of mature females are over twice greater than those of mature males. To obtain sufficient information on gonad differentiation and the relationship between gonad development and growth in the fish, we compared gene expression between the ovary and testis using suppression subtractive hybridization (SSH). Testis cDNAs are subtracted from ovary cDNAs and are used to establish an ovary testis-subtracted cDNA library. A total of 41 nonredundant clones are identified, including 20 known cDNAs, 9 uncharacterized cDNAs (EST clones), and 12 novel sequences. For selected genes such as ZPC, RacGAP, survivin, aquaporin, CPEB, O5, O15, and O18, gene expression patterns are analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR). The results confirm that these genes are only expressed in the ovary and not in the testis, or at higher levels in the ovary than in the testis. At the same time, expressions of certain genes such as ZPC, survivin, aquaporin, CPEB, and O15 are demonstrated to possess sexual dimorphism in the kidney or muscle, and spleen. The results suggest that these genes could play key roles not only in the ovary but in other female tissues as well.
- Cynoglossus semilaevis,
- SSH,
- gene expression,
- sexual dimorphism,
- RT-PCR

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References(28)

References

[1]	Adida, C., Crotty, P.L., Berrebi, M.D. et al. Am. J. Pathol., 152 (1998),pp. 43-49
[2]	Altieri, D.C. Validating survivin as a cancer therapeutic target Nat. Rev. Cancer, 3 (2003),pp. 46-54
[3]	Altieri, D.C. Molecular circuits of apoptosis regulation and cell division control: the survivin paradigm J. Cell Biochem., 92 (2004),pp. 656-663
[4]	Ambrosini, G., Adida, C., Altieri, D.C. Nat. Med., 3 (1997),pp. 917-921
[5]	Arbeitman, M.N., Furlong, E.E.M., Imam, F. et al. Science, 297 (2002),pp. 2270-2275
[6]	Blazquez, M., Bosma, P.T., Fraser, E.J. et al. Fish as models for the neuroendocrine regulation of reproduction and growth Comp. Biochm. Physc., 119 (1998),pp. 345-364
[7]	Bukovsky, A., Chen, T.T., Wimalasena, J. et al. Cellular localization of luteinizing hormone receptor immunoreactivity in the ovaries of immature, gonadotropin-primed and normal cycling rats Biol. Reprod., 48 (1993),pp. 1367-1382
[8]	Chu, S.L., Weng, C.F., Hsiao, C.D. et al. Aquaculture, 251 (2006),pp. 537-548
[9]	Diatchenko, L., Lukyanov, S., Lau, Y.F. et al. Suppression subtractive hybridization: a versatile method for identifying differentially expressed genes Methods Enzymol., 303 (1999),pp. 349-379
[10]	Eppig, J.J., Wigglesworth, K., Pendola, F. The ovarian mammalian oocyte orchestrates the rate of ovarian follicular development Proc. Natl. Acad. Sci. USA, 99 (2002),pp. 2890-2894
[11]	Erickson, G.F., Shimaski, S. The role of the oocyte in folliculogenesis Trends Endocrinol. Metab., 11 (2000),pp. 193-198
[12]	Fabra, M., Raldua, D., Power, D.M. et al. Marine fish egg hydration is aquaporin-mediated Science, 307 (2005),p. 545
[13]	Graaf, A.O., de Witte, T., Jansen, J.H. Inhibitor of apoptosis proteins: new therapeutic targets in hematological cancer? Leukemia, 18 (2004),pp. 1751-1759
[14]	Hake, L.E., Richter, J.D. Cell, 79 (1994),pp. 617-627
[15]	Jin, W., Riley, R.M., Wolfinger, R.D. et al. Nat. Genet., 29 (2001),pp. 389-395
[16]	Kanamori, A., Naruse, K., Mitani, H. et al. Gene, 305 (2003),pp. 35-45
[17]	Kimura, K., Tsuji, T., Takada, Y. et al. Accumulation of GTP-bound RhoA during cytokinesis and a critical role of ECT2 in this accumulation J. Biol. Chem., 275 (2000),pp. 17233-17236
[18]	Lukyanenko, Y., Chen, J.J., Hutson, J.C. Testosterone regulated 25-hydroxycholesterol production in testicular macrophages Biol. Reprod., 67 (2002),pp. 1435-1438
[19]	Madaule, P., Eda, M., Watanabe, N. et al. Role of citron kinase as a target of the small GTPase Rho in cytokinesis Nature, 394 (1998),pp. 491-494
[20]	O'Connell, C.B., Wheatley, S.P., Ahmed, S. et al. The small GTP-binding protein Rho regulates cortical activities in cultured cells during division J. Cell Biol., 144 (1999),pp. 305-313
[21]	Parisi, M., Nuttall, R., Naiman, D. et al. Science, 299 (2003),pp. 697-700
[22]	Ranz, J.M., Castillo-Davis, C.I., Meiklejohn, C.D. et al. Science, 300 (2003),pp. 1742-1745
[23]	Rinn, J.L., Rozowsky, J.S., Laurenzi, I.J. et al. Major molecular differences between mammalian sexes are involved in drug metabolism and renal function Dev. Cell, 6 (2004),pp. 791-800
[24]	Seli, M., Lalioti, M.D., Flaherty, S.M. et al. An embryonic poly(A)-binding protein (ePAB) is expressed in mouse oocytes and early preimplantation embryos Proc. Natl. Acad. Sci. USA, 102 (2005),pp. 367-372
[25]	Stebbins-Boaz, B., Hake, L.E., Richter, J.D. EMBO J., 15 (1996),pp. 2582-2592
[26]	Toure, A., Dorseuil, O., Morin, L. et al. J. Biol. Chem., 273 (1998),pp. 6019-6023
[27]	Van Aelst, L., D'Souza-Schorey, C. Rho GTPases and signaling networks Genes Dev., 11 (1997),pp. 2295-2322
[28]	Wassarman, P.M., Jovine, L., Litscher, E.S. Mouse zona pellucida genes and glycoproteins Cytogenet. Genome Res., 105 (2004),pp. 228-234