Two sequence alterations, a 136 bp InDel and an A/C polymorphic site, in the S5 locus are associated with spikelet fertility of indica-japonica hybrid in rice

Qing Ji; Jufei Lu; Qing Chao; Yan Zhang; Meijing Zhang; Minghong Gu; Mingliang Xu

doi:10.1016/S1673-8527(09)60025-4

Volume 37 Issue 1

Jan. 2010

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Two sequence alterations, a 136 bp InDel and an A/C polymorphic site, in the S5 locus are associated with spikelet fertility of indica-japonica hybrid in rice

doi: 10.1016/S1673-8527(09)60025-4

Qing Ji^{a, #},
Jufei Lu^{b, #},
Qing Chao^a,
Yan Zhang^a,
Meijing Zhang^a,
Minghong Gu^b,
Mingliang Xu^{a, c}

a
National Maize Improvement Center, China Agricultural University, Beijing 100193, China
b
Key lab for plant biotechnology of Jiangsu province, Yangzhou University, Yangzhou 225009, China
c
Key Laboratory of Crop Genetic Improvement, Beijing 100193, China

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Corresponding author: E-mail address: gumh@yzu.edu.cn (Minghong Gu); E-mail address: mxu@cau.edu.cn (Mingliang Xu)
Received Date: 2009-09-13
Accepted Date: 2009-10-21
Rev Recd Date: 2009-10-15

Available Online: 2010-02-18

Publish Date: 2010-01-20

Abstract

Abstract

The rice indica/japonica hybrid shows strong heterosis. However, such inter-subspecific hybrid can't be directly used in rice production due to its low spikelet fertility. The S5 locus was proved to be associated with fertility of indica/japonica hybrid and its S5n allele from wide-compatibility variety (WCV) is capable to overcome fertility barrier. In the present study, we reported the causal sites in the S5 locus responsible for compatibility of indica/japonica hybrid. Fine-mapping of the S5 locus using the 11 test-cross families pinpoints a candidate S5 locus encoding aspartic protease (Asp). Intragenic recombination within the Asp gene happened in a number of recombinants, resulting in chimeric S5j-S5n alleles. Just like S5n, the chimeric S5j-S5n allele displayed higher spikelet fertility when combined with the S5i allele. In the complementary test, however, the S5n allele from WCVs failed to enhance fertilities of the indica/japonica hybrids. Compared to both indica and japonica varieties, all nine WCVs from different resources are characterized with a 136 bp deletion in the Asp N-terminus, which probably renders the S5n allele non-functional. Furthermore, an A/C polymorphic site is detected 1,233 bp downstream of the Asp start codon. The heterozygous A/C site of the Asp gene in indica/japonica hybrid is believed to be the casual factor to cause partial sterility. The functional makers based on the two polymorphic sites will be broadly used in developing wide-compatibility rice varieties.
- rice,
- wide-compatibility

These authors contributed equally to this work.

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References(37)

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