Expression and responses to dehydration and salinity stresses of V-PPase gene members in wheat

Yuezhi Wang; Haibin Xu; Guangxiang Zhang; Huilan Zhu; Lixia Zhang; Zhengzhi Zhang; Caiqin Zhang; Zhengqiang Ma

doi:10.1016/S1673-8527(08)60164-2

Volume 36 Issue 12

Dec. 2009

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Article Navigation > Journal of Genetics and Genomics > 2009 > 36(12): 711-720

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Expression and responses to dehydration and salinity stresses of V-PPase gene members in wheat

doi: 10.1016/S1673-8527(08)60164-2

The Applied Plant Genomics Laboratory, Crop Genomics and Bioinformatics Center & National Key Lab of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China

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Corresponding author: E-mail address: zqm2@njau.edu.cn (Zhengqiang Ma)
Received Date: 2009-02-16
Accepted Date: 2009-05-17
Rev Recd Date: 2009-05-07

Available Online: 2009-12-21

Publish Date: 2009-12-20

Abstract

Abstract

Vacuolar H⁺-translocating pyrophosphatase (V-PPase) is a key enzyme related to plant growth as well as abiotic stress tolerance. In this work, wheat V-PPase genesTaVP1, TaVP2 and TaVP3 were identified. TaVP1 and TaVP2 are more similar to each other than to TaVP3. Their deduced polypeptide sequences preserve the topological structure and essential residues of V-PPases. Phylogenetic studies suggested that monocot plants, at least monocot grasses, have three VP paralogs. TaVP3 transcripts were only detected in developing seeds, and no TaVP2 transcripts were found in germinating seeds. TaVP2 was mainly expressed in shoot tissues and down-regulated in leaves under dehydration. Its expression was up-regulated in roots under high salinity. TaVP1 was relatively more ubiquitously and evenly expressed than TaVP2. Its expression level in roots was highest among the tissues examined, and was inducible by salinity stress. These results indicated that the V-PPase gene paralogs in wheat are differentially regulated spatially and in response to dehydration and salinity stresses.
- wheat,
- abiotic stress,
- phylogenetic analysis

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References(50)

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