Fine mapping quantitative trait loci affecting milk production traits on bovine chromosome 6 in a Chinese Holstein population

Gui Mei; Cengceng Yin; Xiangdong Ding; Qin Zhang

doi:10.1016/S1673-8527(08)60157-5

Volume 36 Issue 11

Nov. 2009

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Article Navigation > Journal of Genetics and Genomics > 2009 > 36(11): 653-660

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Fine mapping quantitative trait loci affecting milk production traits on bovine chromosome 6 in a Chinese Holstein population

doi: 10.1016/S1673-8527(08)60157-5

State Key Laboratory for Agrobiotechnology, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China

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Corresponding author: E-mail address: qzhang@cau.edu.cn (Qin Zhang)
Received Date: 2009-05-21
Accepted Date: 2009-08-14
Rev Recd Date: 2009-07-08

Available Online: 2009-11-20

Publish Date: 2009-11-20

Abstract

Abstract

To fine map the previously detected quantitative trait loci (QTLs) affecting milk production traits on bovine chromosome 6 (BTA6), 15 microsatellite markers situated within an interval of 14.3 cM spanning from BMS690 to BM4528 were selected and 918 daughters of 8 sires were genotyped. Two mapping approaches, haplotype sharing based LD mapping and single marker regression mapping, were used to analyze the data. Both approaches revealed a quantitative trait locus (QTL) with significant effects on milk yield, fat yield and protein yield located in the segment flanked by markers BMS483 and MNB209, which spans a genetic distance of 0.6 cM and a physical distance of 1.5 Mb. In addition, the single marker regression mapping also revealed a QTL affecting fat percentage and protein percentage at marker DIK2291. Our fine mapping work will facilitate the cloning of candidate genes underlying the QTLs for milk production traits.
- fine mapping,
- QTL,
- milk production traits

These authors contributed equally to this work.

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References(30)

References

[1]	Benjamini, Y., Hochberg, Y. Controlling the false discovery rate: A practical and powerful approach to multiple testing J. Roy. Statist. Soc. Ser. B, 57 (1995),pp. 289-300
[2]	Chen, H.Y., Zhang, Q., Yin, C.C. et al. Detection of quantitative trait loci affecting milk production traits on bovine chromosome 6 in a Chinese Holstein population by the daughter design J. Dairy Sci., 89 (2006),pp. 782-790
[3]	Chen, H.Y., Zhang, Q., Wang, C.K. et al. Mapping QTLs on BTA6 affecting milk production traits in a Chinese Holstein population Chinese Sci. Bull., 50 (2005),pp. 1737-1742
[4]	Christians, J.K., Keightley, P.D. Fine mapping of a murine growth locus to a 1.4-cM region and resolution of linked QTL Mamm. Genome, 15 (2004),pp. 482-491
[5]	Cohen-Zinder, M., Seroussi, E., Larkin, D.M. et al. Genome Res., 15 (2005),pp. 936-944
[6]	Ding, X., Zhang, Q., Flury, C. et al. Haplotype reconstruction and estimation of haplotype frequencies from nuclear families with only one parent available Hum. Hered., 62 (2006),pp. 12-19
[7]	Farnir, F., Grisart, B., Coppieters, W. et al. Simultaneous mining of linkage and linkage disequilibrium to fine map quantitative trait loci in outbred half-sib pedigrees: Revisiting the location of a quantitative trait locus with major effect on milk production on bovine chromosome 14 Genetics, 161 (2002),pp. 275-287
[8]	Georges, M., Nielsen, D., Mackinnon, M. et al. Mapping quantitative trait loci controlling milk production in dairy cattle by exploiting progeny testing Genetics, 139 (1995),pp. 907-920
[9]	Grapes, L., Dekkers, J.C., Rothschild, M.F. et al. Comparing linkage disequilibrium-based methods for fine mapping quantitative trait loci Genetics, 166 (2004),pp. 1561-1570
[10]	Harder, B., Bennewitz, J., Reinsch, N. et al. Mapping of quantitative trait loci for lactation persistency traits in German Holstein dairy cattle J. Anim. Breed. Genet., 123 (2006),pp. 89-96
[11]	Ihara, N., Takasuga, A., Mizoshita, K. et al. A comprehensive genetic map of the cattle genome based on 3802 microsatellites Genome Res., 14 (2004),pp. 1987-1998
[12]	Khatkar, M.S., Thomson, P.C., Tammen, I. et al. Linkage disequilibrium on chromosome 6 in Australian Holstein-Friesian cattle Genet. Sel. Evol., 38 (2006),pp. 463-477
[13]	Lapointe, J., Labrie, C. Identification and cloning of a novel androgen-responsive gene, uridine diphosphoglucose dehydrogenase, in human breast cancer cells Endocrinology, 140 (1999),pp. 4486-4493
[14]	Li, Q., Wan, J.M. SSRHunter: Development of a local searching software for SSR sites Hereditas (Beijing), 27 (2005),pp. 808-810
[15]	Lin, B., White, J.T., Utleg, A.G. et al. Isolation and characterization of human and mouse WDR19, a novel WD-repeat protein exhibiting androgen-regulated expression in prostate epithelium Genomics, 82 (2003),pp. 331-342
[16]	Meuwissen, T.H., Karlsen, A., Lien, S. et al. Fine mapping of a quantitative trait locus for twinning rate using combined linkage and linkage disequilibrium mapping Genetics, 161 (2002),pp. 373-379
[17]	Moggs, J.G., Murphy, T.C., Lim, F.L. et al. Anti-proliferative effect of estrogen in breast cancer cells that re-express ERalpha is mediated by aberrant regulation of cell cycle genes J. Mol. Endocrinol., 34 (2005),pp. 535-551
[18]	Nezer, C., Collette, C., Moreau, L. et al. Genetics, 165 (2003),pp. 277-285
[19]	Olsen, H.G., Lien, S., Svendsen, M. et al. Fine mapping of milk production QTL on BTA6 by combined linkage and linkage disequilibrium analysis J. Dairy Sci., 87 (2004),pp. 690-698
[20]	Olsen, H.G., Lien, S., Gautier, M. et al. Mapping of a milk production quantitative trait locus to a 420-kb region on bovine chromosome 6 Genetics, 169 (2005),pp. 275-283
[21]	Riquet, J., Coppieters, W., Cambisano, N. et al. Fine-mapping of quantitative trait loci by identity by descent in outbred populations: Application to milk production in dairy cattle Proc. Natl. Acad. Sci. USA, 96 (1999),pp. 9252-9257
[22]	Ron, M., Kliger, D., Feldmesser, E. et al. Multiple quantitative trait locus analysis of bovine chromosome 6 in the Israeli Holstein population by a daughter design Genetics, 159 (2001),pp. 727-735
[23]	Schnabel, R.D., Kim, J.J., Ashwell, M.S. et al. Fine-mapping milk production quantitative trait loci on BTA6: Analysis of the bovine osteopontin gene Proc. Natl. Acad. Sci. USA, 102 (2005),pp. 6896-6901
[24]	Szyda, J., Liu, Z., Reinhardt, F. et al. Estimation of quantitative trait loci parameters for milk production traits in German Holstein dairy cattle population J. Dairy Sci., 88 (2005),pp. 356-367
[25]	Velmala, R.J., Vilkki, H.J., Elo, K.T. et al. A search for quantitative trait loci for milk production traits on chromosome 6 in Finnish Ayrshire cattle Anim. Genet., 30 (1999),pp. 136-143
[26]	Weikard, R., Kuhn, C., Goldammer, T. et al. The bovine PPARGC1A gene: Molecular characterization and association of an SNP with variation of milk fat synthesis Physiol. Genomics, 21 (2005),pp. 1-13
[27]	Weikard, R., Goldammer, T., Laurent, P. et al. A gene-based high-resolution comparative radiation hybrid map as a framework for genome sequence assembly of a bovine chromosome 6 region associated with QTL for growth, body composition, and milk performance traits BMC Genomics, 7 (2006),p. 53
[28]	Weller, J.I., Song, J.Z., Heyen, D.W. et al. A new approach to the problem of multiple comparisons in the genetic dissection of complex traits Genetics, 150 (1998),pp. 1699-1706
[29]	Zhang, Q., Boichard, D., Hoeschele, I. et al. Mapping quantitative trait loci for milk production and health of dairy cattle in a large outbred pedigree Genetics, 149 (1998),pp. 1959-1973
[30]	Zhao, H.H., Fernando, R.L., Dekkers, J.C. Power and precision of alternate methods for linkage disequilibrium mapping of quantitative trait loci Genetics, 175 (2007),pp. 1975-1986