Effects of age on segregation of the X and Y chromosomes in cultured lymphocytes from Chinese men

Yaxian Song; Qian Chen; Zhen Zhang; Heli Hou; Ding Zhang; Qinghua Shi

doi:10.1016/S1673-8527(08)60136-8

Volume 36 Issue 8

Aug. 2009

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Article Navigation > Journal of Genetics and Genomics > 2009 > 36(8): 467-474

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Effects of age on segregation of the X and Y chromosomes in cultured lymphocytes from Chinese men

doi: 10.1016/S1673-8527(08)60136-8

a
Laboratory of Molecular and Cell Genetics, Hefei National Laboratory for Physical Sciences at Microscale, School of Life Sciences, University of Science and Technology of China, Hefei 230027, China
b
Department of Science of Biology, Bengbu Medical Collage, Bengbu 233000, China

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Corresponding author: E-mail address: qshi@ustc.edu.cn (Qinghua Shi)
Received Date: 2009-02-13
Accepted Date: 2009-06-02
Rev Recd Date: 2009-06-01

Available Online: 2009-08-14

Publish Date: 2009-08-20

Abstract

Abstract

Chromosome malsegregation in binucleated lymphocytes is a useful endpoint to evaluate age effect on genetic stability. However, theinvestigations on chromosome malsegregation in binucleated lymphocytes from Chinese are scarce. In this study, peripheral blood lymphocytes were collected from 14 old (60–70 years) and 10 young (22–26 years) healthy Chinese men. To detect malsegregation of the sexchromosomes, multi-color fluorescence in situ hybridization (FISH) was performed on binucleated lymphocytes, cytokinesis-blocked bycytochalasin B at the first mitosis after phytohaemagglutinin stimulation. Compared with that in young men, a significant increase in frequencies of loss of chromosome X (9.2 ± 3.2‰vs. 1.1 ± 0.9‰, P < 0.001) and Y (2.5 ± 1.9‰ vs. 0.2 ± 0.3‰, P < 0.001) was found inold men. Similarly, nondisjunction of chromosome X (16.5 ± 3.4‰ vs. 3.5 ± 1.1‰, P < 0.001) and Y (7.2 ± 2.6‰ vs. 2.4 ± 1.3‰, P < 0.001) occurred more frequently in old men than in young men. Regardless of donor's age, nondisjunction is more prevalent than loss forboth chromosome X and Y. The frequencies of observed simultaneous malsegregation were relatively higher than the expected, suggesting an association between malsegregation. These results indicated that in Chinese men, malsegregation of the sex chromosomes increases with age in an associated fashion, and nondisjunction accounts for the majority of spontaneous chromosome malsegregation.
- chromosome loss,
- nondisjunction,
- chromosome X,
- chromosome Y,
- lymphocyte,
- Chinese men

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References(39)

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