Cytogenetic and molecular identification of three Triticum aestivum-Leymus racemosus translocation addition lines

Le Wang; Jianhua Yuan; Tongde Bie; Bo Zhou; Peidu Chen

doi:10.1016/S1673-8527(08)60127-7

Volume 36 Issue 6

Jun. 2009

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Article Navigation > Journal of Genetics and Genomics > 2009 > 36(6): 379-385

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Cytogenetic and molecular identification of three Triticum aestivum-Leymus racemosus translocation addition lines

doi: 10.1016/S1673-8527(08)60127-7

Le Wang^a,
Jianhua Yuan^{a, b},
Tongde Bie^{a, c},
Bo Zhou^a,
Peidu Chen^a

a
National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China
b
Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
c
Yangzhou Academy of Agricultural Sciences, Yangzhou 225007, China

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Corresponding author: E-mail address: pdchen@njau.edu.cn (Peidu Chen)
Received Date: 2008-05-20
Accepted Date: 2009-01-22
Rev Recd Date: 2009-01-21

Available Online: 2009-06-16

Publish Date: 2009-06-20

Abstract

Abstract

Chromosome 2C from Aegilops cylindrica has the ability to induce chromosome breakage in common wheat (Tritivum aestivum). In the BC₁F₃ generation of the T. aestivum cv. Chinese Spring and a hybrid between T. aestivum-Leymus racemosus Lr.7 addition line and T. aestivum-Ae. cylindrica 2C addition line, three disomic translocation addition lines (2n = 44) were selected by mitotic chromosome C-banding and genomic in situ hybridization. We further characterized these T. aestivum-L. racemosus translocation addition lines, NAU636, NAU637 and NAU638, by chromosome C-banding, in situ hybridization using the A- and D-genome-specific bacterial artificial chromosome (BAC) clones 676D4 and 9M13; plasmids pAs1 and pSc119.2, and 45S rDNA; as well as genomic DNA of L. racemosus as probes, in combination with double ditelosomic test cross and SSR marker analysis. The translocation chromosomes were designated as T3AS-Lr7S, T6BS-Lr7S, and T5DS-Lr7L. The translocation line T3AS-Lr7S was highly resistant to Fusarium head blight and will be useful germplasm for resistance breeding.
- wheat,
- translocation line,
- C-banding,
- test cross analysis

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References(19)

References

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