Isolation and characterization of a sterile-dwarf mutant in Asian cotton (Gossypium arboreum L.)

Chuntai Wu; Baoliang Zhou; Tianzhen Zhang

doi:10.1016/S1673-8527(08)60123-X

Volume 36 Issue 6

Jun. 2009

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Article Navigation > Journal of Genetics and Genomics > 2009 > 36(6): 343-353

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Isolation and characterization of a sterile-dwarf mutant in Asian cotton (Gossypium arboreum L.)

doi: 10.1016/S1673-8527(08)60123-X

National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, China

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Corresponding author: E-mail address: cotton@njau.edu.cn (Tianzhen Zhang)
Received Date: 2008-08-27
Accepted Date: 2009-02-24
Rev Recd Date: 2008-11-25

Available Online: 2009-06-16

Publish Date: 2009-06-20

Abstract

Abstract

Plant height is an important trait in cotton. To elucidate the molecular mechanisms of the dwarf phenotype, a sterile-dwarf mutant derived from Gossypium arboreum L. cv. Jinhuazhongmian was developed by ⁶⁰Co γ-ray irradiation. The results demonstrated that the steriledwarf mutant phenotype was controlled by a pair of recessive gene, which was designated . Plants carrying the gene contained lower levels of indole-3-acetic acid (IAA) and abscisic acid (ABA) compared with wild-type (WT) plants. The chlorophyll content and net photosynthetic rate in mutant leaves were markedly decreased. However, it was possible that ABA biosynthesis or signaling was involved in governing the phenotype. Semi-quantitative RT-PCR analysis detected 13 differentially expressed ESTs, and the sterile-dwarf mutant exhibited decreased expression levels relative to the WT. The role of nine potential hormone biosynthetic genes in the synthesis of IAA, ABA, polyamines (PAs) and jasmonic acid (JA) were discussed.
- auxin,
- abscisic acid,
- ESTs

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References(39)

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