Root and shoot traits responses to phosphorus deficiency and QTL analysis at seedling stage using introgression lines of rice

Junzhou Li; Yan Xie; Anyong Dai; Lifeng Liu; Zichao Li

doi:10.1016/S1673-8527(08)60104-6

Volume 36 Issue 3

Mar. 2009

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Article Navigation > Journal of Genetics and Genomics > 2009 > 36(3): 173-183

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Root and shoot traits responses to phosphorus deficiency and QTL analysis at seedling stage using introgression lines of rice

doi: 10.1016/S1673-8527(08)60104-6

Key Laboratory of Crop Genomics and Genetic Improvement of Ministry of Agriculture and Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China

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Corresponding author: E-mail address: lizichao@cau.edu.cn (Zichao Li)
Received Date: 2008-10-03
Accepted Date: 2008-12-09
Rev Recd Date: 2008-12-09

Available Online: 2009-03-18

Publish Date: 2009-03-20

Abstract

Abstract

Phosphorous (P) deficiency is a major restraint factor for crop production and plants have developed several mechanisms to adapt to low P stress. In this study, a set of 271 introgression lines (ILs) were used to characterize the responses of seedlings to low P availability and to identify QTLs for root traits, biomass, and plant height under P-deficiency and P-sufficiency conditions. Plant height, total dry weight, shoot dry weight, and root number were inhibited under P-deficiency, whereas maximum root length (MRL) and root-shoot ratio (RS) were induced by P-deficiency stress. Relative MRL (RMRL, the ratio of MRL under P-deficiency to MRL under P-sufficiency condition) and relative RS (RRS) were used to evaluate P-deficiency tolerance at the seedling stage. A total of 24 additive QTLs and 29 pairs of epistatic QTLs were detected, but only qRN4 was detected in both conditions. This suggested that different mechanisms may exist in both P supply levels. QTLs for adaptive traits (RMRL, RRS, RRV, and RRDW) and qRN4 consistently expressed to increase trait stability may contribute to P-deficiency tolerance. Twelve intervals were cluster regions of QTLs for P-deficiency tolerance, and one QTL (qRRS8) showed pleiotropic effects on P-deficiency tolerance and drought tolerance. These interesting QTLs can be used in marker-assisted breeding through the target ILs.
- introgression lines,
- phosphorous deficiency tolerance,
- QTL mapping,
- rice,
- root traits

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References(27)

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