Differentiation potential of bone marrow mesenchymal stem cells in duck

Linfeng Li; Xiujuan Bai; Xuelian Gong; Hongkun Liu; Lina Chen; Weijun Guan; Yuehui Ma

doi:10.1016/S1673-8527(08)60100-9

Volume 36 Issue 3

Mar. 2009

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Article Navigation > Journal of Genetics and Genomics > 2009 > 36(3): 133-140

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Differentiation potential of bone marrow mesenchymal stem cells in duck

doi: 10.1016/S1673-8527(08)60100-9

a
Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193, China
b
College of Animal Science and Technology, Northeast Agricultural University, Harbin 150000, China

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Corresponding author: E-mail address: wjguan86@126.com (Weijun Guan); E-mail address: yuehui.ma@263.net (Yuehui Ma)
Received Date: 2008-09-20
Accepted Date: 2009-01-23
Rev Recd Date: 2009-01-21

Available Online: 2009-03-18

Publish Date: 2009-03-20

Abstract

Abstract

The bone marrow mesenchymal stem cells (MSCs) are multipotent stem cells which can differentiate into mesenchymal cellsin vitro. In this study, MSCs in duck were isolated from bone marrow by density gradient centrifuge separation, purified and expanded in the medium. The primary MSCs were expanded for passages. The different-passage MSCs were induced to differentiate into osteoblasts and neuron-like cells. Karyotype analysis indicated that MSCs kept diploid condition and the hereditary feature was stable. The different-passage MSCs expressed CD44, ICAM- and SSEA-4, but not CD34, CD45 and SSEA-when detected by immunofluorescence staining. There was no significant difference among the positive rates of passages 2, 6 and 8 (P > 0.05), but a significant difference existed among those of passages 2, 6, 8 and 11 ( P < 0.05). After the osteogenic inducement was added, the induced different-passage MSCs expressed high-level alkaline phosphatase (ALP), and are positive for tetracycline staining, Alizarin Red staining and Von Kossa staining. After the neural inducement was added, about 70% cells exhibited typical neuron-like phenotype, the induced different-passage MSCs expressed Nestin, neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) when detected by immunofluorescence staining. There was no significant difference among the positive rates of passages 3, 4 and 6 ( P>0.05), but a significant difference existed among those of passages 3, 4, 6 and 8 (P<0.05). These results suggest that MSCs in duck were capable of differentiating into osteoblasts and neuron-like cellsin vitro.
- duck,
- mesenchymal stem cells,
- induction,
- differentiation

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References(13)

References

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