Characterization of Arabidopsis MYB transcription factor gene AtMYB17 and its possible regulation by LEAFY and AGL15

Yunfei Zhang; Guangyu Cao; Li-Jia Qu; Hongya Gu

doi:10.1016/S1673-8527(08)60096-X

Volume 36 Issue 2

Feb. 2009

Turn off MathJax

Article Contents

Article Navigation > Journal of Genetics and Genomics > 2009 > 36(2): 99-107

PDF( 980 KB)

Characterization of Arabidopsis MYB transcription factor gene AtMYB17 and its possible regulation by LEAFY and AGL15

doi: 10.1016/S1673-8527(08)60096-X

National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China

More Information

Corresponding author: E-mail address: guhy@pku.edu.cn (Hongya Gu)
Received Date: 2008-12-02
Accepted Date: 2008-12-04
Rev Recd Date: 2008-12-03

Available Online: 2009-02-13

Publish Date: 2009-02-20

Abstract

Abstract

MYB transcription factors compose one of the largest transcription factor families in Arabidopsis, which play important roles in various developmental processes as well as defense responses against environmental stresses. In this study, we report the characterization of AtMYB17 gene, a putative R2R3 type MYB gene family member in Arabidopsis. AtMYB17 was found exclusively localized in nuclear, with an activation domain at its C-terminus. AtMYB17 was highly expressed in inflorescences and siliques, especially at early flower developmental stages. The level of AtMYB17 transcripts was also found to increase after imbibition during seed germination and gradually concentrate to the shoot apex. Bioinformatics analysis identified several binding sites of LEAFY (LFY) and AGL15 in the promoter region of AtMYB17. Promoter-GUS fusion analysis showed that the LFY binding sites were important in fine-tuning regulation of the spatio-temporal expression of AtMYB17 in transgenic plants. Moreover, AtMYB17 was up-regulated in 35S::AGL15 plants. Taken together, our data suggest that LFY may be involved in the regulation of AtMYB17, possibly together with AGL15, and thereafter in early inflorescence development and seed germination.
- transcription factor,
- LEAFY,
- AGL15

FullText(HTML)

References(39)

References

[1]	Avila, J., Nieto, C., Cana, L. et al. Plant J. (1993),pp. 553-562
[2]	Blazquez, M.A., Soowal, L.N., Lee, I. et al. Development, 124 (1997),pp. 3835-3844
[3]	Bowman, J.L., Alvarez, J., Weigel, D. et al. Development, 119 (1993),pp. 721-743
[4]	Busch, M.A., Bomblies, K., Weigel, D. Science, 285 (1999),pp. 585-587
[5]	Chen, Y., Yang, X., He, K. et al. Plant Mol. Biol., 60 (2006),pp. 107-124
[6]	Elliott, R.C., Betzner, A.S., Huttner, E. et al. Plant Cell, 8 (1996),pp. 155-168
[7]	Fang, S.C., Fernandez, D.E. Effect of regulated overexpression of the MADS domain factor AGL15 on flower senescence and fruit maturation Plant Physiol., 130 (2002),pp. 78-89
[8]	Fernandez, D.E., Heck, G.R., Perry, S.E. et al. The embryo MADS domain factor AGL15 acts postembryonically: Inhibition of perianth senescence and abscission via constitutive expression Plant Cell, 12 (2000),pp. 183-198
[9]	Guo, L., Wang, Z.Y., Lin, H. et al. Expression and functional analysis of the rice plasma-membrane intrinsic protein gene family Cell Res., 16 (2006),pp. 277-286
[10]	Huala, E., Sussex, I.M. Plant Cell, 4 (1992),pp. 901-913
[11]	Jin, H., Martin, C. Plant Mol. Biol., 41 (1999),pp. 577-585
[12]	Klucher, K.M., Chow, H., Reiser, L. et al. Plant Cell, 8 (1996),pp. 137-153
[13]	Kong, Z., Perry, S. Control of expression and autoregulation of AGL15, a member of the MADS-box family Plant J., 41 (2005),pp. 583-594
[14]	Kranz, H.D., Denekamp, M., Greco, R. et al. Plant J., 16 (1998),pp. 263-276
[15]	Krizek, B.A., Prost, V., Macias, A. AINTEGUMENTA promotes petal identity and acts as a negative regulator of AGAMOUS Plant Cell, 12 (2000),pp. 1357-1366
[16]	Lamb, R.S., Hill, T.A., Tan, Q.K. et al. Development, 129 (2002),pp. 2079-2086
[17]	Lee, I., Wolfe, D.S., Nilsson, O. et al. Curr. Biol., 7 (1997),pp. 95-104
[18]	Li, J., Li, X., Guo, L. et al. J. Exp. Bot., 57 (2006),pp. 1263-1273
[19]	Liu, Z., Franks, R.G., Klink, V.P. Regulation of gynoecium marginal tissue formation by LEUNIG and AINTEGUMENTA Plant Cell, 12 (2000),pp. 1879-1891
[20]	Martin, C., Bhatt, K., Baumann, K. et al. The mechanics of cell fate determination in petals Philos. Trans. R. Soc. Lond., B, Biol. Sci., 357 (2002),pp. 809-813
[21]	Meissner, R.C., Jin, H., Cominelli, E. et al. Plant Cell, 11 (1999),pp. 1827-1840
[22]	Mizukami, Y., Fischer, R.L. Proc. Natl. Acad. Sci. USA, 97 (2000),pp. 942-947
[23]	Nilsson, O., Lee, I., Blazquez, M.A. et al. Flowering-time genes modulate the response to LEAFY activity Genetics, 150 (1998),pp. 403-410
[24]	Noda, K.I., Glover, B.J., Linstead, P. et al. Flower color intensity depends on specialized cell shape controlled by a Myb-related transcription factor Nature, 369 (1994),pp. 661-664
[25]	Parcy, F., Nilsson, O., Busch, M.A. et al. A genetic framework for floral patterning Nature, 395 (1998),pp. 561-566
[26]	Payne, T., Clement, J., Arnold, D. et al. Development, 126 (1999),pp. 671-682
[27]	Qu, L.-J., Zhu, X.Y. Curr. Opin. Plant Biol., 9 (2006),pp. 544-549
[28]	Riechmann, J.L., Heard, J., Martin, G. et al. Science, 290 (2000),pp. 2105-2110
[29]	Schultz, E.A., Haughn, G.W. Plant Cell, 3 (1991),pp. 771-781
[30]	Smyth, D.R., Bowman, J.L., Meyerowitz, E.M. Plant Cell, 2 (1990),pp. 755-767
[31]	Stracke, R., Werber, M., Weisshaar, B. Curr. Opin. Plant Biol., 4 (2001),pp. 447-456
[32]	Tang, W., Perry, S.E. J. Biol. Chem., 278 (2003),pp. 28154-28159
[33]	Wagner, D., Sablowski, R.W., Meyerowitz, E.M. Transcriptional activation of APETALA1 by LEAFY Science, 285 (1999),pp. 582-584
[34]	Wang, H., Caruso, L.V., Downie, A.B. et al. The embryo MADS domain protein AGAMOUS-Like 15 directly regulates expression of a gene encoding an enzyme involved in gibberellin metabolism Plant Cell, 16 (2004),pp. 1206-1219
[35]	Weigel, D., Alvarez, J., Smyth, D.R. et al. Cell, 69 (1992),pp. 843-859
[36]	William, D.A., Su, Y., Smith, M.R. et al. Genomic identification of direct target genes of LEAFY Proc. Natl. Acad. Sci. USA, 101 (2004),pp. 1775-1780
[37]	Ye, R., Yao, Q.H., Xu, Z.H. et al. Development of an efficient method for the isolation of factors involved in gene transcription during rice embryo development Plant J., 38 (2004),pp. 348-357
[38]	Yi, L., Qu, L.-J., Chang, S. et al. Two nuclear localization signals required for the nuclear localization of rice ribosomal proteins S4 Plant Sci., 162 (2002),pp. 251-256
[39]	Zhu, C., Perry, S.E. Control of expression and autoregulation of AGL15, a member of the MADS-box family Plant J., 41 (2005),pp. 583-594